A NEW ENZYMATIC CYCLING TECHNIQUE FOR GLUTAMATE DETERMINATION IN BRAIN MICRODIALYSATES

被引：53

作者：

MITANI, A

KUBO, H

IGA, K

IMON, H

KADOYA, F

KATAOKA, K

机构：

[1] EHIME UNIV,SCH MED,DEPT SURG,SHIGENOBU,EHIME 79102,JAPAN

[2] EHIME UNIV,SCH MED,DEPT ORTHOPED,SHIGENOBU,EHIME 79102,JAPAN

[3] EHIME UNIV,SCH MED,DEPT PHYSIOL REPROD,SHIGENOBU,EHIME 79102,JAPAN

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 02期

关键词：

Enzymatic cycling; Glutamate; Hippocampus; Microdialysis; Mongolian gerbil;

D O I：

10.1111/j.1471-4159.1990.tb01930.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative analysis of glutamate in brain dialysate was made by using an enzymatic cycling technique. This method made it possible to measure the concentration of glutamate in dialysate collected at 30‐s intervals. Dialysates were collected from Mongolian gerbil hippocampus before, during, and after two 90‐s ischemic insults at an interval of 5 min. An extracellular increase in levels of glutamate was already observed in samples collected during a 30–60 s period after the onset of each ischemia, and the levels of glutamate were maximal at the end of each period of ischemia (approximately a fourfold increase). The increased levels of glutamate rapidly returned almost to preischemic levels by 30 s of recirculation. This method will provide more precise information about temporal changes in the extracellular glutamate concentration in the brain during ischemia. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：709 / 711

页数：3

共 9 条

[1] ELEVATION OF THE EXTRACELLULAR CONCENTRATIONS OF GLUTAMATE AND ASPARTATE IN RAT HIPPOCAMPUS DURING TRANSIENT CEREBRAL-ISCHEMIA MONITORED BY INTRACEREBRAL MICRODIALYSIS [J].