DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR CAFFEINE

被引：13

作者：

FICKLING, SA ^{[1
]}

HAMPTON, SM ^{[1
]}

TEALE, D ^{[1
]}

MIDDLETON, BA ^{[1
]}

MARKS, V ^{[1
]}

机构：

[1] ST LUKES HOSP,DEPT CLIN BIOCHEM & NUTR,GUILDFORD GU1 3WT,SURREY,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 129卷 / 02期

关键词：

Caffeine; Cross-reactivity; ELISA;

D O I：

10.1016/0022-1759(90)90435-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A competitive enzyme-linked immunosorbent assay suitable for the measurement of caffeine in plasma and serum has been developed. Sheep immunised with an immunogen prepared by coupling 7-(5-carboxypenthyl)1,3-dimethylxanthine to egg albumin produced antibodies with little crossreactivity with the metabolites of caffeine. The enzyme label was prepared by coupling 7-(5-carboxypentyl)-1,3-dimethylxanthine to peroxidase using the mixed anhydride method. The assay, which has a sensitivity of 0.01 μmol/1, permits direct measurement of caffeine in plasma and serum samples. 50 plasma samples measured by ELISA and by an established radioimmunoassay showed a correlation of r = 0.97 (P < 0.001). © 1990.

引用

页码：159 / 164

页数：6

共 6 条

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[5] DETERMINATION OF CAFFEINE, THEOPHYLLINE AND THEOBROMINE IN SERUM AND SALIVA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SCOTT, NR
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