HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HISTAMINE N-METHYLTRANSFERASE ACTIVITY

被引：21

作者：

FUKUDA, H

YAMATODANI, A

IMAMURA, I

MAEYAMA, K

WATANABE, T

WADA, H

机构：

[1] OSAKA UNIV,FAC MED,DEPT PHARMACOL 2,NAKANOSHIMA 4-3-57,KITA KU,OSAKA 530,JAPAN

[2] TOHOKU UNIV,SCH MED,DEPT PHARMACOL 1,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 567卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80152-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with o-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product, N-tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification.

引用

页码：459 / 464

页数：6

共 13 条

[1] INHIBITION AND ACTIVATION OF HISTAMINE METHYLTRANSFERASE BY METHYLATED HISTAMINES [J].