CHEMILUMINESCENCE DOT BLOT HYBRIDIZATION ASSAY FOR DETECTION OF B19 PARVOVIRUS DNA IN HUMAN SERA

被引：40

作者：

MUSIANI, M ^{[1
]}

ZERBINI, M ^{[1
]}

GIBELLINI, D ^{[1
]}

GENTILOMI, G ^{[1
]}

VENTUROLI, S ^{[1
]}

GALLINELLA, G ^{[1
]}

FERRI, E ^{[1
]}

GIROTTI, S ^{[1
]}

机构：

[1] UNIV BOLOGNA,IST SCI CHIM,I-40126 BOLOGNA,ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 09期

关键词：

D O I：

10.1128/JCM.29.9.2047-2050.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.

引用

页码：2047 / 2050

页数：4

共 36 条

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