SIMPLE NONRADIOACTIVE DNA HYBRIDIZATION METHOD FOR IDENTIFICATION OF SIBLING SPECIES OF ANOPHELES DIRUS (DIPTERA, CULICIDAE) COMPLEX

被引：14

作者：

AUDTHO, M ^{[1
]}

TASSANAKAJON, A ^{[1
]}

BOONSAENG, V ^{[1
]}

TPIANKIJAGUM, S ^{[1
]}

PANYIM, S ^{[1
]}

机构：

[1] CHULALONGKORN UNIV,FAC SCI,DEPT BIOCHEM,BANGKOK 10330,THAILAND

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 1995年 / 32卷 / 02期

关键词：

ANOPHELES DIRUS; SIBLING SPECIES IDENTIFICATION; NONRADIOACTIVE DETECTION;

D O I：

10.1093/jmedent/32.2.107

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A simple method was developed for species identification of mosquitoes in the Anopheles dirus complex found in Thailand using horseradish peroxidase-labeled DNA probes and a chemiluminescent detection system. Species-specific DNA probes for Anopheles dirus, species B, C, and D detected 1-5 ng of target DNA, a sensitivity that was comparable vith the radioisotopic detection system. Identification of individual mosquitoes was performed by dot-blot analysis of crude mosquito DNA. The method allowed identification using third or fourth instars as well as the adult head, thorax, or abdomen. This technique sucessfully identified the sibling species of the A. dirus complex in field collections.

引用

页码：107 / 111

页数：5

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