PREPARATION AND CHARACTERIZATION OF HUMAN INTERLEUKIN-5 EXPRESSED IN RECOMBINANT ESCHERICHIA-COLI

被引：42

作者：

PROUDFOOT, AEI ^{[1
]}

FATTAH, D ^{[1
]}

KAWASHIMA, EH ^{[1
]}

BERNARD, A ^{[1
]}

WINGFIELD, PT ^{[1
]}

机构：

[1] GLAXO GRP RES LTD,GREENFORD UB6 0HE,MIDDX,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1990年 / 270卷 / 02期

关键词：

D O I：

10.1042/bj2700357

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for human interleukin-5 was synthesized and expressed in Escherichia coli under control of a heat-inducible promoter. High-level expression, 10-15% of total cellular protein, was achieved in E. coli. The protein was produced in an insoluble state. A simple extraction, renaturation and purification scheme is described. The recombinant protein was found to be a homodimer, similar to the natural murine-derived protein. Despite the lack of glycosylation, high specific activities were obtained in three 'in vitro' biological assays. Physical characterization of the protein showed it to be mostly α-helical, supporting the hypothesis that a conformational similarity exists among certain cytokines.

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页码：357 / 361

页数：5

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