FPLC PURIFICATION OF MOUSE MONOCLONAL-ANTIBODIES FROM ASCITIC FLUID USING BLUE DEAE AND THIOPHILIC SORBENTS

被引：14

作者：

JURONEN, E ^{[1
]}

PARIK, J ^{[1
]}

TOOMIK, P ^{[1
]}

机构：

[1] ESTAR BIOTECHNOL DEV CO,ESTONIAN BIOCTR,TARTU 202400,ESTONIA,USSR

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 136卷 / 01期

关键词：

CHROMATOGRAPHY; FAST PROTEIN LIQUID; MONOCLONAL ANTIBODY PURIFICATION; BLUE DEAE GEL; THIOPHILIC ADSORPTION;

D O I：

10.1016/0022-1759(91)90255-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two fast methods for the purification of mouse monoclonal antibodies from ascites fluids using Blue-DEAE and 'thiophilic' absorbent (T gel) in the FPLC system are described. Blue-DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory absorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.

引用

页码：103 / 109

页数：7

共 10 条

[1] A ONE-STEP PURIFICATION METHOD FOR MONOCLONAL-ANTIBODIES BASED ON SALT-PROMOTED ADSORPTION CHROMATOGRAPHY ON A THIOPHILIC ADSORBENT
BELEW, M
JUNTTI, N
LARSSON, A
PORATH, J
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 102 (02) : 173 - 182
[2] ONE-STEP PURIFICATION OF MOUSE MONOCLONAL-ANTIBODIES FROM ASCITIC FLUID BY DEAE AFFI-GEL BLUE CHROMATOGRAPHY
BRUCK, C
PORTETELLE, D
GLINEUR, C
BOLLEN, A
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 53 (03) : 313 - 319
[3] A SIMPLIFICATION OF HEUKESHOVEN AND DERNICKS SILVER STAINING OF PROTEINS
DAMERVAL, C
LEGUILLOUX, M
BLAISONNEAU, J
DEVIENNE, D
[J]. ELECTROPHORESIS, 1987, 8 (03) : 158 - 159
[4] PROTEIN-PURIFICATION USING IMMOBILIZED TRIAZINE DYES
DEAN, PDG
WATSON, DH
[J]. JOURNAL OF CHROMATOGRAPHY, 1979, 165 (03): : 301 - 319
[5] PURIFICATION OF MURINE IGG3 AND IGM MONOCLONAL-ANTIBODIES BY EUGLOBULIN PRECIPITATION
GARCIAGONZALEZ, M
BETTINGER, S
OTT, S
OLIVIER, P
KADOUCHE, J
POULETTY, P
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 111 (01) : 17 - 23
[6] PURIFICATION OF IGM MONOCLONAL-ANTIBODY FROM MURINE ASCITIC FLUID BY A 2-STEP COLUMN CHROMATOGRAPHY PROCEDURE
JOHNSON, E
MIRIBEL, L
ARNAUD, P
TSANG, KY
[J]. IMMUNOLOGY LETTERS, 1987, 14 (02) : 159 - 165
[7] JUNGBAUER A, 1989, MONOCLONAL ANTIBODIE, P161
[8] RAPID, SIMPLE AND SENSITIVE ANTIGEN CAPTURE ELISA FOR THE QUANTITATION OF MYOGLOBIN USING MONOCLONAL-ANTIBODIES
JURONEN, EI
VIIKMAA, MH
MIKELSAAR, AVN
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 111 (01) : 109 - 115
[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[10] THIOPHILIC ADSORPTION - A NEW METHOD FOR PROTEIN FRACTIONATION
PORATH, J
MAISANO, F
BELEW, M
[J]. FEBS LETTERS, 1985, 185 (02) : 306 - 310

← 1 →