NUCLEIC-ACID DETECTION SYSTEMS FOR ENTEROVIRUSES

被引:49
作者
ROTBART, HA
机构
关键词
D O I
10.1128/CMR.4.2.156-168.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The enteroviruses comprise nearly 70 human pathogens responsible for a wide array of diseases including poliomyelitis, meningitis, myocarditis, and neonatal sepsis. Current diagnostic tests for the enteroviruses are limited in their use by the slow growth, or failure to grow, of certain serotypes in culture, the antigenic diversity among the serotypes, and the low titer of virus in certain clinical specimens. Within the past 6 years, applications of molecular cloning techniques, in vitro transcription vectors, automated nucleic acid synthesis, and the polymerase chain reaction have resulted in significant progress toward nucleic acid-based detection systems for the enteroviruses that take advantage of conserved genomic sequences across many, if not all, serotypes. Similar approaches to the study of enteroviral pathogenesis have already produced dramatic advances in our understanding of how these important viruses cause their diverse clinical spectra.
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页码:156 / 168
页数:13
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