DEVELOPMENT OF A CLONING ASSAY WITH HIGH CLONING EFFICIENCY TO DETECT INDUCTION OF 6-THIOGUANINE-RESISTANT LYMPHOCYTES IN SPLEEN OF ADULT MICE FOLLOWING IN-VIVO INHALATION EXPOSURE TO 1,3-BUTADIENE

被引：54

作者：

TATES, AD

VANDAM, FJ

DEZWART, FA

VANTEYLINGEN, CMM

NATARAJAN, AT

机构：

[1] MGC-Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, State University of Leiden, 2333 AL Leiden

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1994年 / 309卷 / 02期

关键词：

1,3-BUTADIENE; HPRT MUTATION; 6-THIOGUANINE-RESISTANT T-LYMPHOCYTES; MOUSE; IN VIVO;

D O I：

10.1016/0027-5107(94)90106-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cloning assay with high cloning efficiency has been developed to detect spontaneous and induced 6-thioguanine-resistant T-lymphocytes (HPRT mutants) from the spleen of adult mice. The mean cloning efficiency in untreated male mice of 20-22 weeks old was 34.5 +/- 11.2% (SD) and the corresponding mutant frequency 0.7 +/- 0.8 (SD) x 10(-6). The cloning efficiencies obtained in this study are substantially higher than those reported previously by other investigators. Using this assay, it could be demonstrated that inhalation exposure of mice to 200, 500 or 1300 ppm of 1,3-butadiene for 6 h/day on 5 consecutive days caused a statistically significant induction of 6-thioguanine-resistant mutations in T-lymphocytes from spleens of adult mice exposed to 1300 ppm. The exposure to 1300 ppm resulted in a three-fold increase of the spontaneous mutant frequency. The mutant frequency after exposure to 500 ppm was higher than the control but the increase was not significant.

引用

页码：299 / 306

页数：8

共 19 条

[1] MUTAGENICITY OF 1,3-BUTADIENE INHALATION IN SOMATIC AND GERMINAL CELLS OF MICE [J].

ADLER, ID ;

CAO, J ;

FILSER, JG ;

GASSNER, P ;

KESSLER, W ;

KLIESCH, U ;

NEUHAUSERKLAUS, A ;

NUSSE, M .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1994, 309 (02) :307-314

[2] QUANTITATIVE ASSAY FOR MUTATION IN DIPLOID HUMAN-LYMPHOBLASTS USING MICROTITER PLATES [J].

FURTH, EE ;

THILLY, WG ;

PENMAN, BW ;

LIBER, HL ;

RAND, WM .

ANALYTICAL BIOCHEMISTRY, 1981, 110 (01) :1-8

[3] FACTORS THAT AFFECT THE FREQUENCY OF THIOGUANINE-RESISTANT LYMPHOCYTES IN MICE FOLLOWING EXPOSURE TO ETHYLNITROSOUREA [J].

JONES, IM ;

BURKHARTSCHULTZ, K ;

STROUT, CL ;

CRIPPEN, TL .

ENVIRONMENTAL MUTAGENESIS, 1987, 9 (03) :317-329

[4] A METHOD TO QUANTIFY SPONTANEOUS AND INVIVO INDUCED THIOGUANINE-RESISTANT MOUSE LYMPHOCYTES [J].

JONES, IM ;

BURKHARTSCHULTZ, K ;

CARRANO, AV .

MUTATION RESEARCH, 1985, 147 (03) :97-105

[5] CLONED MOUSE LYMPHOCYTES PERMIT ANALYSIS OF SOMATIC MUTATIONS THAT OCCUR INVIVO [J].

JONES, IM ;

BURKHARTSCHULTZ, K ;

CRIPPEN, TL .

SOMATIC CELL AND MOLECULAR GENETICS, 1987, 13 (04) :325-333

[6] XENOGENEIC MONOCLONAL ANTIBODIES TO MOUSE LYMPHOID DIFFERENTIATION ANTIGENS [J].

LEDBETTER, JA ;

HERZENBERG, LA .

IMMUNOLOGICAL REVIEWS, 1979, 47 :63-90

[7] MUTATION ASSAY AT THE THYMIDINE KINASE LOCUS IN DIPLOID HUMAN-LYMPHOBLASTS [J].

LIBER, HL ;

THILLY, WG .

MUTATION RESEARCH, 1982, 94 (02) :467-485

[8] RESPONSES OF THE L5178Y MOUSE LYMPHOMA FORWARD MUTATION ASSAY .5. GASES AND VAPORS [J].

MCGREGOR, D ;

BROWN, AG ;

CATTANACH, P ;

EDWARDS, I ;

MCBRIDE, D ;

RIACH, C ;

SHEPHERD, W ;

CASPARY, WJ .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1991, 17 (02) :122-129

[9] DETERMINATION OF MUTAGENICITY IN TISSUES OF TRANSGENIC MICE FOLLOWING EXPOSURE TO 1,3-BUTADIENE AND N-ETHYL-N-NITROSOUREA [J].

RECIO, L ;

OSTERMANGOLKAR, S ;

CSANADY, GA ;

TURNER, MJ ;

MYHR, B ;

MOSS, O ;

BOND, JA .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1992, 117 (01) :58-64

[10] MUTATIONAL SPECTRUM AT THE HPRT LOCUS IN SPLENIC T-CELLS OF B6C3F(1) MICE EXPOSED TO N-ETHYL-N-NITROSOUREA [J].

SKOPEK, TR ;

WALKER, VE ;

COCHRANE, JE ;

CRAFT, TR ;

CARIELLO, NF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (17) :7866-7870

← 1 2 →