ENZYME-KINETICS BY MIDINFRARED SPECTROSCOPY - BETA-FRUCTOSIDASE STUDY BY A ONE-STEP ASSAY

被引：22

作者：

CADET, F ^{[1
]}

PIN, FW ^{[1
]}

ROUCH, C ^{[1
]}

ROBERT, C ^{[1
]}

BARET, P ^{[1
]}

机构：

[1] FAC SCI ST DENIS,LA REUNION CTICS,FRANCE DOM LAB CHIM ORGAN,RECH & DEV LAB,F-97464 ST DENIS,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1995年 / 1246卷 / 02期

关键词：

MID-FTIR; ATTENUATED TOTAL REFLECTION; PRINCIPAL COMPONENT REGRESSION; ENZYME KINETICS; BETA-FRUCTOSIDASE;

D O I：

10.1016/0167-4838(94)00193-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An alternate method for enzyme study is proposed. Multidimensional statistical analysis applied on mid-infrared attenuated total reflectance spectra (Cadet et al. (1991) Appl. Spectrosc. 42, 166-172) collected during a kinetic allows a direct and fast quantification of the remaining substrate, as well as a one step enzymatic assay. Furthermore, the combination of these techniques may be used as a structural tool. The method applied to the study of beta-fructosidase is developed in this paper as an example. With appropriate calibration, the method may be extend to any enzyme.

引用

页码：142 / 150

页数：9

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