Monoclonal antibodies specific for the organophosphate pesticide azinphos-methyl

被引：13

作者：

Jones, WT ^{[1
]}

Harvey, D ^{[1
]}

Jones, SD ^{[1
]}

Ryan, GB ^{[1
]}

Wynberg, H ^{[1
]}

TenHoeve, W ^{[1
]}

Reynolds, PHS ^{[1
]}

机构：

[1] UNIV GRONINGEN,GRONINGEN,NETHERLANDS

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 1995年 / 7卷 / 01期

关键词：

azinphos-methyl; organophosphate; monoclonal antibodies; ELISA;

D O I：

10.1080/09540109509354861

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

2-(2-Mercapto-5-methyl-1,3,2-dioxaphosphorinan-5-yl,2-sulphide) methoxyacetic acid has been synthesized and used to prepare an azinphos hapten and protein conjugates. Monoclonal antibodies of high affinity against the pesticide azinphos-methyl were prepared from mice immunized with the hapten-ovalbumin conjugate. The best monoclonal antibody could be used in an indirect enzyme-linked immunosorbent assay (ELISA) to quantify azinphos methyl at concentrations from 50 pg ml(-1) to 2 ng ml(-1) (2.5-100 pg/assay). The corresponding limit of detection of the pesticide was less than 40 pg ml(-1). Only the closely related pesticide azinphos-ethyl showed any appreciable cross-reactivity in this assay.

引用

页码：9 / 19

页数：11

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