COMPARATIVE STUDIES ON BHC METABOLIZING ENZYMES DDT DEHYDROCHLORINASE AND GLUTATHIONE S-TRANSFERASES

It was demonstrated by gel filtration that the enzymes from house flies for the metabolisms of α- and γ-1,2,3,4,5,6-hexachlorocyclohexane, γ- and -δ2,3,4,5,6-pentachloro- cyclohex-1-ene, 2,3,4,5,6-pentachlorobenzyl chloride, 1-chloro-2,4-dinitrobenzene, 2,3,4,5,6- pentachloro-β-nitrostyrene and 1,1,1-trichloro-2,2-di-(p-chlorophenyl) ethane were indistingishable from one another in their molecular sizes which were estimated at 36,000 to 38,000 by using reference proteins. The same result was obtained with rat liver homogenate. Two house fly isozymes were separated by GM-cellulose column chromatography. The house fly enzymes were clearly separated from rat liver enzymes by electrophoresis, which also revealed the presence of 2 isozymes in the rat liver homogenate. © 1968 Taylor and Francis Group LLC.