THE SYNTHESIS OF A D-AMINO-ACID ESTER IN AN ORGANIC MEDIA WITH ALPHA-CHYMOTRYPSIN MODIFIED BY A BIO-IMPRINTING PROCEDURE

被引：63

作者：

STAHL, M ^{[1
]}

MANSSON, MO ^{[1
]}

MOSBACH, K ^{[1
]}

机构：

[1] CHEM CTR LUND,POB 124,S-22100 LUND,SWEDEN

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 03期

关键词：

D O I：

10.1007/BF01026792

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this report the use of chymotrypsin in the synthesis of a D-amino acid ester is described. The enzyme was modified to accept the D-form of a tryptophane derivative, by precipitating the enzyme-inhibitor complex between chymotrypsin and N-acetyl-D-tryptophane in 1-propanol. The precipitate was then used to synthesize N-acetyl-D-tryptophane ethyl ester in cyclhexane. The rate of the D-esterification was 7.5 nmole/mg enzyme·h. © 1990 Kluwer Academic Publishers.

引用

页码：161 / 166

页数：6

共 11 条

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