AUTOMETALLOGRAPHIC SILVER-ENHANCEMENT OF COLLOIDAL GOLD PARTICLES USED TO LABEL PHAGOCYTIC-CELLS

The present paper demonstrates that colloidal gold silver-enhanced by autometallography (AMG) can be used to label phagocytic cells for light microscopic detection. Cultured macrophages were exposed to 0.5-mu-l 6 nm colloidal gold particles for 24 or 48 h. Other cultures were exposed to 25-mu-l of the same solution for 1 to 14 days. The staining was found to be stable also when new unmarked cells were applied. The colloidal gold had no adverse effect on the cells. The presented technique might also prove valuable for estimation of the total number of phagocytes in a culture or in an organism by applying labelled cells to culture or organism, and to ascertain the fate of a population of marked cells.