A SIMPLE METHOD FOR THE PURIFICATION OF MITOCHONDRIAL-DNA FROM PLASMODIUM-FALCIPARUM

被引：5

作者：

FLORES, MV ^{[1
]}

STEWART, TS ^{[1
]}

OSULLIVAN, WJ ^{[1
]}

机构：

[1] UNIV NEW S WALES,SCH BIOCHEM,KENSINGTON,NSW 2033,AUSTRALIA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1991年 / 21卷 / 05期

关键词：

PLASMODIUM-FALCIPARUM; MITOCHONDRIA; DNA; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0020-7519(91)90067-H

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Mitochondrial DNA (mtDNA) from Plasmodium falciparum was isolated by conventional differential centrifugation in an SS34 rotor, a simpler method than CsCl centrifugation of total DNA as employed by other workers. The nature of the sample was verified by sequencing a polymerase chain reaction (PCR) product obtained using oligonucleotide primers derived from known malarial mtDNA sequence.

引用

页码：605 / 608

页数：4

共 18 条

[1] FURTHER-STUDIES AND ELECTRON-MICROSCOPIC CHARACTERIZATION OF PLASMODIUM-BERGHEI DNA [J].

DORE, E ;

FRONTALI, C ;

FORTE, T ;

FRATARCANGELI, S .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1983, 8 (04) :339-352

[2] A CIRCULAR DNA IN MALARIA PARASITES ENCODES AN RNA-POLYMERASE LIKE THAT OF PROKARYOTES AND CHLOROPLASTS [J].

GARDNER, MJ ;

WILLIAMSON, DH ;

WILSON, RJM .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 44 (01) :115-123

[3] MITOCHONDRIAL-DNA OF THE HUMAN MALARIAL PARASITE PLASMODIUM-FALCIPARUM [J].

GARDNER, MJ ;

BATES, PA ;

LING, IT ;

MOORE, DJ ;

MCCREADY, S ;

GUNASEKERA, MBR ;

WILSON, RJM ;

WILLIAMSON, DH .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 31 (01) :11-17

[4] PYRIMIDINE DENOVO SYNTHESIS DURING THE LIFE-CYCLE OF THE INTRAERYTHROCYTIC STAGE OF PLASMODIUM-FALCIPARUM [J].

GERO, AM ;

BROWN, GV ;

OSULLIVAN, WJ .

JOURNAL OF PARASITOLOGY, 1984, 70 (04) :536-541

[5] THE ESTABLISHMENT OF GENOMIC DNA LIBRARIES FOR THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM AND IDENTIFICATION OF INDIVIDUAL CLONES BY HYBRIDIZATION [J].

GOMAN, M ;

LANGSLEY, G ;

HYDE, JE ;

YANKOVSKY, NK ;

ZOLG, JW ;

SCAIFE, JG .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1982, 5 (06) :391-400

[6] CONVERSION OF DIHYDROOROTATE TO OROTATE IN PARASITIC PROTOZOA [J].

GUTTERIDGE, WE ;

DAVE, D ;

RICHARDS, WHG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 582 (03) :390-401

[7]

HEERY DM, 1990, TRENDS GENET, V6, P173

[8] PYRIMIDINE NUCLEOTIDE BIOSYNTHESIS IN ANIMALS - GENES, ENZYMES, AND REGULATION OF UMP BIOSYNTHESIS [J].

JONES, ME .

ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 :253-279

[9] MITOCHONDRIA AS THE SITE OF ACTION OF TETRACYCLINE ON PLASMODIUM-FALCIPARUM [J].

KIATFUENGFOO, R ;

SUTHIPHONGCHAI, T ;

PRAPUNWATTANA, P ;

YUTHAVONG, Y .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 34 (02) :109-115

[10] CIRCULAR MITOCHONDRIAL-DNA FROM AVIAN MALARIAL PARASITE PLASMODIUM-LOPHURAE [J].

KILEJIAN, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 390 (03) :276-284

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