FAILURE OF ULTRA-VIOLET IRRADIATION AND AUTOCLAVING TO ELIMINATE PCR CONTAMINATION

被引：26

作者：

DWYER, DE ^{[1
]}

SAKSENA, N ^{[1
]}

机构：

[1] INST PASTEUR,UNITE ONCOL VIRALE,DOCTEUR ROUX,F-75724 PARIS 15,FRANCE

来源：

MOLECULAR AND CELLULAR PROBES | 1992年 / 6卷 / 01期

关键词：

D O I：

10.1016/0890-8508(92)90075-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：87 / 88

页数：2

共 9 条

[1] MORE FALSE-POSITIVE PROBLEMS [J].

CIMINO, GD ;

METCHETTE, K ;

ISAACS, ST ;

ZHU, YS .

NATURE, 1990, 345 (6278) :773-774

[2]

KITCHIN PA, 1990, NATURE, V344, P20

[3] AVOIDING FALSE POSITIVES WITH PCR [J].

KWOK, S ;

HIGUCHI, R .

NATURE, 1989, 339 (6221) :237-238

[4]

LO Y, 1990, LANCET, V335, P679

[5] USE OF URACIL DNA GLYCOSYLASE TO CONTROL CARRY-OVER CONTAMINATION IN POLYMERASE CHAIN-REACTIONS [J].

LONGO, MC ;

BERNINGER, MS ;

HARTLEY, JL .

GENE, 1990, 93 (01) :125-128

[6] DNA AMPLIFICATION FOR DIRECT DETECTION OF HIV-1 IN DNA OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS [J].

OU, CY ;

KWOK, S ;

MITCHELL, SW ;

MACK, DH ;

SNINSKY, JJ ;

KREBS, JW ;

FEORINO, P ;

WARFIELD, D ;

SCHOCHETMAN, G .

SCIENCE, 1988, 239 (4837) :295-297

[7] NESTED POLYMERASE CHAIN-REACTION ASSAY FOR THE DETECTION OF CYTOMEGALOVIRUS OVERCOMES FALSE POSITIVES CAUSED BY CONTAMINATION WITH FRAGMENTED DNA [J].

PORTERJORDAN, K ;

ROSENBERG, EI ;

KEISER, JF ;

GROSS, JD ;

ROSS, AM ;

NASIM, S ;

GARRETT, CT .

JOURNAL OF MEDICAL VIROLOGY, 1990, 30 (02) :85-91

[8] SHEDDING LIGHT ON PCR CONTAMINATION [J].

SARKAR, G ;

SOMMER, SS .

NATURE, 1990, 343 (6253) :27-27

[9] MORE LIGHT ON PCR CONTAMINATION [J].

SARKAR, G ;

SOMMER, S .

NATURE, 1990, 347 (6291) :340-341

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