INVIVO PHOSPHORYLATION OF THE 30-KDA PROTEIN OF TOBACCO MOSAIC-VIRUS

被引：54

作者：

WATANABE, Y ^{[1
]}

OGAWA, T ^{[1
]}

OKADA, Y ^{[1
]}

机构：

[1] KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,PCC TECHNOL INC,TOCHIGI LAB,TOCHIGI 32914,JAPAN

来源：

FEBS LETTERS | 1992年 / 313卷 / 02期

关键词：

30-KDA PROTEIN; PHOSPHORYLATION; CELL-TO-CELL MOVEMENT; INVITRO TRANSCRIPT; TOBACCO MOSAIC VIRUS; TOBACCO PROTOPLAST;

D O I：

10.1016/0014-5793(92)81440-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 30-kDa protein of tobacco mosaic virus, which is involved in cell-to-cell movement function, is phosphorylated in tobacco protoplasts. To investigate which portion of the protein is phosphorylated we inoculated several truncated 30-kDa protein mutants into protoplasts and determined whether or not those truncated proteins are phosphorylated. The results showed that amino acid residues 234-261 of the 30-kDa protein are required for this phosphorylation.

引用

页码：181 / 184

页数：4

共 25 条

[1]

Atabekov J G, 1990, Adv Virus Res, V38, P201, DOI 10.1016/S0065-3527(08)60863-5

[2] EXPRESSION OF THE TOBACCO MOSAIC-VIRUS MOVEMENT PROTEIN USING A BACULOVIRUS EXPRESSION VECTOR [J].

ATKINS, D ;

ROBERTS, K ;

HULL, R ;

PREHAUD, C ;

BISHOP, DHL .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :2831-2835

[3] THE TOBACCO MOSAIC VIRUS-30K MOVEMENT PROTEIN IN TRANSGENIC TOBACCO PLANTS IS LOCALIZED TO PLASMODESMATA [J].

ATKINS, D ;

HULL, R ;

WELLS, B ;

ROBERTS, K ;

MOORE, P ;

BEACHY, RN .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :209-211

[4] THE TMV MOVEMENT PROTEIN - ROLE OF THE C-TERMINAL 73 AMINO-ACIDS IN SUBCELLULAR-LOCALIZATION AND FUNCTION [J].

BERNA, A ;

GAFNY, R ;

WOLF, S ;

LUCAS, WJ ;

HOLT, CA ;

BEACHY, RN .

VIROLOGY, 1991, 182 (02) :682-689

[5] THE P-30 MOVEMENT PROTEIN OF TOBACCO MOSAIC-VIRUS IS A SINGLE-STRAND NUCLEIC-ACID BINDING-PROTEIN [J].

CITOVSKY, V ;

KNORR, D ;

SCHUSTER, G ;

ZAMBRYSKI, P .

CELL, 1990, 60 (04) :637-647

[6] VISUALIZATION AND CHARACTERIZATION OF TOBACCO MOSAIC-VIRUS MOVEMENT PROTEIN-BINDING TO SINGLE-STRANDED NUCLEIC-ACIDS [J].

CITOVSKY, V ;

WONG, ML ;

SHAW, AL ;

PRASAD, BVV ;

ZAMBRYSKI, P .

PLANT CELL, 1992, 4 (04) :397-411

[7] PLANT-VIRUS MOVEMENT PROTEINS [J].

DEOM, CM ;

LAPIDOT, M ;

BEACHY, RN .

CELL, 1992, 69 (02) :221-224

[8] THE 30-KILODALTON GENE-PRODUCT OF TOBACCO MOSAIC-VIRUS POTENTIATES VIRUS MOVEMENT [J].

DEOM, CM ;

OLIVER, MJ ;

BEACHY, RN .

SCIENCE, 1987, 237 (4813) :389-394

[9] EFFECTS OF TERMINAL DELETION MUTATIONS ON FUNCTION OF THE MOVEMENT PROTEIN OF TOBACCO MOSAIC-VIRUS [J].

GAFNY, R ;

LAPIDOT, M ;

BERNA, A ;

HOLT, CA ;

DEOM, CM ;

BEACHY, RN .

VIROLOGY, 1992, 187 (02) :499-507

[10]

HULL R, 1989, ANNU REV PHYTOPATHOL, V27, P213

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