POSSIBLE INVOLVEMENT OF NITRIC-OXIDE IN ACETYLCHOLINE-INDUCED INCREASE OF INTRACELLULAR CA2+ CONCENTRATION AND CATECHOLAMINE RELEASE IN BOVINE ADRENAL CHROMAFFIN CELLS

被引：34

作者：

UCHIYAMA, Y ^{[1
]}

MORITA, K ^{[1
]}

KITAYAMA, S ^{[1
]}

SUEMITSU, T ^{[1
]}

MINAMI, N ^{[1
]}

MIYASAKO, T ^{[1
]}

DOHI, T ^{[1
]}

机构：

[1] HIROSHIMA UNIV,SCH DENT,DEPT PHARMACOL,MINAMI KU,HIROSHIMA 734,JAPAN

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 1994年 / 65卷 / 01期

关键词：

NITRIC OXIDE; CATECHOLAMINE; CHROMAFFIN CELL;

D O I：

10.1254/jjp.65.73

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The role of nitric oxide (NO) in neurotransmitter release was studied using bovine adrenal medullary chromaffin cells. L-Arginine and sodium nitroprusside (SNP) slightly increased the intracellular free calcium concentration ([Ca2+](i)), and the effects of the agents were dependent on the presence of the extracellular Ca2+ ([Ca2+](o)), but were not blocked by verapamil (30 mu M) or diltiazem (30 mu M). SNP enhanced the acetylcholine (ACh)-induced rise in [Ca2+](i) in the presence but not in the absence of [Ca2+](o). The effects of L-arginine but not those of SNP were inhibited by N-omega-nitro-L-arginine (L-NNA). L-NNA significantly reduced the ACh-induced rise in [Ca2+](i) and catecholamine (CA) release, and the reduction was restored by L-arginine but not by D-arginine. These results suggest a possible involvement of NO in ACh-induced [Ca2+](i) rise and CA release in bovine adrenal chromaffin cells.

引用

页码：73 / 77

页数：5

共 15 条

[1] LOCALIZATION OF NITRIC-OXIDE SYNTHASE INDICATING A NEURAL ROLE FOR NITRIC-OXIDE [J].

BREDT, DS ;

HWANG, PM ;

SNYDER, SH .

NATURE, 1990, 347 (6295) :768-770

[2] CLONED AND EXPRESSED NITRIC-OXIDE SYNTHASE STRUCTURALLY RESEMBLES CYTOCHROME-P-450 REDUCTASE [J].

BREDT, DS ;

HWANG, PM ;

GLATT, CE ;

LOWENSTEIN, C ;

REED, RR ;

SNYDER, SH .

NATURE, 1991, 351 (6329) :714-718

[3] EFFECT OF SODIUM-AZIDE ON CATECHOLAMINE RELEASE FROM ISOLATED ADRENAL-GLAND AND ON GUANYLATE-CYCLASE [J].

DOHI, T ;

MORITA, K ;

TSUJIMOTO, A .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1983, 94 (3-4) :331-335

[4] FUNCTIONAL AND MORPHOLOGICAL CHARACTERIZATION OF ISOLATED BOVINE ADRENAL-MEDULLARY CELLS [J].

FENWICK, EM ;

FAJDIGA, PB ;

HOWE, NBS ;

LIVETT, BG .

JOURNAL OF CELL BIOLOGY, 1978, 76 (01) :12-30

[5] GLUTAMATE, NITRIC-OXIDE AND CELL CELL SIGNALING IN THE NERVOUS-SYSTEM [J].

GARTHWAITE, J .

TRENDS IN NEUROSCIENCES, 1991, 14 (02) :60-67

[6] MECHANISM OF CYCLIC-AMP FACILITATION OF STIMULATION-EVOKED CATECHOLAMINE RELEASE IN ADRENAL CHROMAFFIN CELLS .2. INHIBITION OF NA+,K+-ATPASE BY CYCLIC-AMP [J].

MORITA, K ;

DOHI, T ;

MINAMI, N ;

KITAYAMA, S ;

TSUJIMOTO, A .

NEUROCHEMISTRY INTERNATIONAL, 1991, 19 (1-2) :81-85

[7] MECHANISM OF CYCLIC-AMP FACILITATION OF STIMULATION-EVOKED CATECHOLAMINE RELEASE IN ADRENAL CHROMAFFIN CELLS .1. EVIDENCE FOR ENHANCEMENT OF EVOKED INCREASE IN CYTOSOLIC FREE NA+ CONCENTRATION BY CYCLIC-AMP ELEVATION [J].

MORITA, K ;

DOHI, T ;

KITAYAMA, S ;

TSUJIMOTO, A .

NEUROCHEMISTRY INTERNATIONAL, 1991, 19 (1-2) :71-79

[8] INVOLVEMENT OF CA-2+ ENTRY AND INOSITOL TRISPHOSPHATE-INDUCED INTERNAL CA-2+ MOBILIZATION IN MUSCARINIC RECEPTOR-MEDIATED CATECHOLAMINE RELEASE IN DOG ADRENAL CHROMAFFIN CELLS [J].

OHTSUKI, H ;

MORITA, K ;

MINAMI, N ;

SUEMITSU, T ;

TSUJIMOTO, A ;

DOHI, T .

NEUROCHEMISTRY INTERNATIONAL, 1992, 21 (02) :259-267

[9]

OKA M, 1982, ADV BIOSCI, V36, P29

[10] CYCLIC-GMP REGULATES NICOTINE-INDUCED SECRETION FROM CULTURED BOVINE ADRENAL CHROMAFFIN CELLS - EFFECTS OF 8-BROMO-CYCLIC GMP, ATRIAL-NATRIURETIC-PEPTIDE, AND NITROPRUSSIDE (NITRIC-OXIDE) [J].

OSULLIVAN, AJ ;

BURGOYNE, RD .

JOURNAL OF NEUROCHEMISTRY, 1990, 54 (05) :1805-1808

← 1 2 →