FUCOSYLATED GLYCOSPHINGOLIPIDS OF HUMAN MYELOID CELLS

被引:15
作者
KONG, RKM
BARRIOS, A
KNAPP, W
MACHER, BA
机构
[1] SAN FRANCISCO STATE UNIV,DEPT CHEM & BIOCHEM,SAN FRANCISCO,CA 94132
[2] UNIV VIENNA,INST IMMUNOL,DEPT INTERNAL MED,A-1090 VIENNA,AUSTRIA
关键词
D O I
10.1006/abbi.1993.1094
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Our efforts to determine the carbohydrate binding specificity of two myeloid-specific monoclonal antibodies (VIM-1 and VIM-10) resulted in the purification of three fucosylated glycosphingolipids from human chronic myelogenous leukemia cells. After repeated high-performance liquid chromatographic separations, two forms of fucosylated glycosphingolipids were resolved. VIM-1 and VIM-10 were found to bind to the heptaosylceramide Galβ1-4 (Fucα-1-3)GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer with the Le(x) (Lewis X) epitope, but not to either the hexaosylceramide Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glcβ1-1-Cer or the octaosylceramide Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1 -1-Cer, each with the Le(y) (Lewis Y) epitope. The latter two glycosphingolipids are the first Le(y) antigens to be purified from human leukocytes and structurally characterized. Binding studies with a range of glycosphingolipids from myeloid cells and other biological sources demonstrated that VIM-1 and VIM-10 bind to Le(x) glycosphingolipids with five or more sugar residues, but not to a glycosphingolipid (III3Fuc-nLc6Cer) with an internal Le(x) trisaccharide. © 1993 Academic Press, Inc.
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页码:677 / 683
页数:7
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