CLONING AND CHARACTERIZATION OF THE HUMAN 5,10-METHENYLTETRAHYDROFOLATE SYNTHETASE-ENCODING CDNA

被引：22

作者：

DAYAN, A

BERTRAND, R

BEAUCHEMIN, M

CHAHLA, D

MAMO, A

FILION, M

SKUP, D

MASSIE, B

JOLIVET, J

机构：

[1] HOP HOTEL DIEU,CTR RECH,MONTREAL,PQ H2W 1T8,CANADA

[2] UNIV MONTREAL,INST CANC MONTREAL,CTR RECH LOUIS CHARLES SINARD,MONTREAL,PQ H2L 4M1,CANADA

[3] UNIV MONTREAL,DEPT PATHOL,MONTREAL,PQ H3C 3J7,CANADA

[4] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,MONTREAL,PQ H4P 2R2,CANADA

来源：

GENE | 1995年 / 165卷 / 02期

关键词：

LEUCOVORIN; FOLATE-DEPENDENT COENZYME; 5-FLUOROURACIL;

D O I：

10.1016/0378-1119(95)00321-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Methenyltetrahydrofolate synthetase (MTHFS) catalyses the obligatory initial metabolic step in the intracellular conversion of 5-formyltetrahydrofolate to other reduced folates, We have isolated and sequenced a human MTHFS cDNA which is 872-bp long and codes for a 203-amino-acid protein of 23229 Da. Escherichia coli BL21(DE3), transfected with pET11c plasmids containing an open reading frame encoding MTHFS, showed a 100-fold increase in MTHFS activity in bacterial extracts after IPTG induction. Northern blot studies of human tissues determined that the MTHFS mRNA was expressed preferentially in the liver and Southern blot analysis of human genomic DNA suggested the presence of a single-copy gene.

引用

页码：307 / 311

页数：5

共 31 条

[1] STRUCTURAL ORGANIZATION OF THE MURINE GENE ENCODING NAD-DEPENDENT METHYLENETETRAHYDROFOLATE DEHYDROGENASE-METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE [J].