EARLY PHASES IN IN-VITRO CULTURE OF TOMATO COTYLEDONS - STARCH ACCUMULATION AND PROTEIN PATTERN IN RELATION TO THE HORMONAL TREATMENT

被引：25

作者：

BRANCA, C ^{[1
]}

TORELLI, A ^{[1
]}

FERMI, P ^{[1
]}

ALTAMURA, MM ^{[1
]}

BASSI, M ^{[1
]}

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO BIOL VEGETALE,ROME,ITALY

来源：

PROTOPLASMA | 1994年 / 182卷 / 1-2期

关键词：

IN VITRO CULTURE; TOMATO; STARCH ACCUMULATION; PROTEIN PATTERN;

D O I：

10.1007/BF01403689

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Tomato cotyledon explants, cultured in vitro in the presence of sucrose, were subjected to different hormonal treatments to establish whether the induction of different organogenic programmes could be correlated with differences in starch accumulation and protein electrophoretic pattern. The cytohistological changes in explants over the first 15 days of culture were studied by light and electron microscopy. It was found that starch accumulation occurs under air conditions, though varying in duration and amount. Over the first 2 days of culture the protein electrophoretic pattern changes in a similar way under all conditions, while after 7 days changes take place which are probably related to the different developmental programmes induced by the treatments.

引用

页码：59 / 64

页数：6

共 20 条

[1] Structure and development of the tobacco leaf [J].

Avery, GS .

AMERICAN JOURNAL OF BOTANY, 1933, 20 (09) :565-592

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] AUXIN STRUCTURE AND ACTIVITY ON TOMATO MORPHOGENESIS INVITRO AND PEA STEM ELONGATION [J].

BRANCA, C ;

BUCCI, G ;

DOMIANO, P ;

RICCI, A ;

TORELLI, A ;

BASSI, M .

PLANT CELL TISSUE AND ORGAN CULTURE, 1991, 24 (02) :105-114

[4]

COLEMAN WK, 1977, CAN J BOT, V54, P2704

[5] SOMACLONAL VARIATION VERSUS CHEMICALLY-INDUCED MUTAGENESIS IN TOMATO (LYCOPERSICON-ESCULENTUM L) [J].

GAVAZZI, G ;

TONELLI, C ;

TODESCO, G ;

ARREGHINI, E ;

RAFFALDI, F ;

VECCHIO, F ;

BARBUZZI, G ;

BIASINI, MG ;

SALA, F .

THEORETICAL AND APPLIED GENETICS, 1987, 74 (06) :733-738

[6]

HAWKESFORD MJ, 1991, PLANTA, V185, P323, DOI 10.1007/BF00201051

[7] SOMATIC EMBRYOGENESIS IN SUGARCANE (SACCHARUM-OFFICINARUM L) - GROWTH AND PLANT-REGENERATION FROM EMBRYOGENIC CELL-SUSPENSION CULTURES [J].

HO, WJ ;

VASIL, IK .

ANNALS OF BOTANY, 1983, 51 (06) :719-726

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

MANGAT BS, 1990, PHYSIOL PLANTARUM, V79, P267

[10] SUPPRESSION OF SHOOT FORMATION IN CULTURED TOBACCO CELLS BY GIBBERELLIC ACID [J].

MURASHIGE, T .

SCIENCE, 1961, 134 (347) :280-&

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