SOLUBLE FACTORS INCLUDING PROTEINASES RELEASED FROM DAMAGED CELLS MAY TRIGGER THE WOUND-HEALING PROCESS

被引：16

作者：

TSUBOI, K

YAMAOKA, S

MAKI, M

OHSHIO, G

TOBE, T

HATANAKA, M

机构：

[1] KYOTO UNIV,INST VIRUS RES,KAWAHARA CHO,SAKYO KU,KYOTO 606,JAPAN

[2] KYOTO UNIV,FAC MED,DEPT SURG 1,KYOTO 606,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91151-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The wound healing process is initiated as soon as tissue is injured. Herein, we demonstrate that c-fos and c-myc mRNA transcripts are promptly increased in the wounded tissue in vivo and in vitro. A buffer solution from scraped serum-starved quiescent fibroblasts, when added to resting fibroblasts, caused the increase of c-fos and c-myc mRNA among the indicator cells. Soluble factors contained in the wounding supernatant are responsible for these phenomena and we call them wounding factors. Addition of proteinase inhibitors to the culture medium drastically reduced the c-fos mRNA induction by the wounding factors. Exogenously added trypsin or thrombin mimicked the activity of wounding factors. These results suggest that wounding causes soluble factors including various proteinases to be released from the damaged cells, which trigger the adjacent cells to respond to the injury. © 1990.

引用

页码：1163 / 1170

页数：8

共 19 条

[1] FUNCTIONAL-ROLE FOR C-MYC IN MITOGENIC RESPONSE TO PLATELET-DERIVED GROWTH-FACTOR [J].

ARMELIN, HA ;

ARMELIN, MCS ;

KELLY, K ;

STEWART, T ;

LEDER, P ;

COCHRAN, BH ;

STILES, CD .

NATURE, 1984, 310 (5979) :655-660

[2] PROTEOLYTIC ENZYMES INITIATING CELL DIVISION AND ESCAPE FROM CONTACT INHIBITION OF GROWTH [J].

BURGER, MM .

NATURE, 1970, 227 (5254) :170-&

[3] MITOGENIC ACTIVITY OF BLOOD COMPONENTS .1. THROMBIN AND PROTHROMBIN [J].

CHEN, LB ;

BUCHANAN, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (01) :131-135

[4] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[5] FBJ MURINE OSTEO-SARCOMA VIRUS - IDENTIFICATION AND MOLECULAR-CLONING OF BIOLOGICALLY-ACTIVE PROVIRAL DNA [J].

CURRAN, T ;

PETERS, G ;

VANBEVEREN, C ;

TEICH, NM ;

VERMA, IM .

JOURNAL OF VIROLOGY, 1982, 44 (02) :674-682

[6] TOPOINHIBITION AND SERUM REQUIREMENT OF TRANSFORMED AND UNTRANSFORMED CELLS [J].

DULBECCO, R .

NATURE, 1970, 227 (5260) :802-&

[7] NEW EVIDENCE THAT GROWTH IN 3T3 CELL-CULTURES IS A DIFFUSION-LIMITED PROCESS [J].

DUNN, GA ;

IRELAND, GW .

NATURE, 1984, 312 (5989) :63-65

[8]

FAVERA R D, 1982, Nature (London), V299, P61, DOI 10.1038/299061a0

[9] STIMULATION OF 3T3 CELLS INDUCES TRANSCRIPTION OF THE C-FOS PROTO-ONCOGENE [J].

GREENBERG, ME ;

ZIFF, EB .

NATURE, 1984, 311 (5985) :433-438

[10] CELL-SPECIFIC REGULATION OF THE C-MYC-GENE BY LYMPHOCYTE MITOGENS AND PLATELET-DERIVED GROWTH-FACTOR [J].

KELLY, K ;

COCHRAN, BH ;

STILES, CD ;

LEDER, P .

CELL, 1983, 35 (03) :603-610

← 1 2 →