NOVEL MICROSURGICAL MODEL OF EXPERIMENTAL VASCULAR NEOINTIMAL HYPERPLASIA

被引：4

作者：

TENNANT, M

BARKER, A

STORRIE, AE

MCGEACHIE, JK

机构：

[1] Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Western Australia

[2] Princess Margaret Hospital, Subiaco, Western Australia

[3] Research Centre, Royal Perth Hospital, Perth, Western Australia

来源：

MICROSURGERY | 1993年 / 14卷 / 02期

关键词：

D O I：

10.1002/micr.1920140204

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Neointimal hyperplasia is a common finding after trauma to blood vessels and also as a primary change in atherosclerosis. In this study we have developed a simple model, using microsurgical techniques, for the initiation of neointimal hyperplasia in the rat In 24 Wistar rats, a 2 mm-diameter arteriotomy in the aorta was repaired with a ''patch'' of iliolumbar vein, using eight evenly spaced 10-0 Ethilon sutures. The patch overlapped the edges of the arteriotomy, and the sutures fastened the patch to the subjacent aorta. At 2, 6 and 12 weeks after surgery, the venous patch grafts and segments of the adjacent aortae of eight rats were removed. One-half of the specimens were analyzed by scanning electron microscopy and the other one-half by light microscopy. All patch grafts were surgically successful. Endothelial cells regenerated to cover the patch within 2 weeks of insertion. By 6 weeks after surgery, neointimal hyperplasia, consisting predominantly of smooth muscle, had developed in all patches to a thickness that was not significantly different from that of the adjacent aorta. These findings are consistent with data from other more complex experimental models of neointimal hyperplasia in the rat. We consider that this venous patch technique is a simple but effective model for the initiation of neointimal hyperplasia in the rat and may easily be used to study the experimental effects of various injurious or therapeutic agents on neointimal hyperplasia.

引用

页码：102 / 106

页数：5

共 14 条

[1] Dobrin PB, Littooy FN, Golan J, Blackeman B, Fareed J, Mechanical and histological changes in cannine vein grafts, J Surg Res, 44, pp. 259-265, (1988)
[2] Dilley RJ, (1986)
[3] Ross R, The pathogenesis of atherosclerosis—An update, N Engl J Med, 314, pp. 488-499, (1986)
[4] Dilley RJ, McGeachie JK, Tennant M, The role of cell proliferation and migration in vein to artery graft intimal hyperplasia in rats, Cell Tissue Res, 269, pp. 281-287, (1992)
[5] Prendergast FJ, McGeachie JK, Fabre JW, Winearls CG, Morris PJ, Vein allografts for arterial replacement in rats, Transplantation, 27, pp. 49-54, (1979)
[6] Tennant M, McGeachie JK, A computerised morphometric technique for the analysis of intimal hyperplasia, J Anat, 178, pp. 119-126, (1991)
[7] Storrie EA, McGeachie JK, Samuels R, Hunneybun B, Bartlett N, Transverse or longitudinal arteriotomies in end‐to‐side microvascular anastomoses for small vessels (1–2 mm), Microsurgery, 11, pp. 108-113, (1990)
[8] Zimmerman MJ, McGeachie JK, Quantitation of the relationship between aortic endothelial intercellular cleft morphology and permeability to albumin, Atherosclerosis, 59, pp. 277-282, (1986)
[9] McGeachie JK, Prendergast FJ, Morris PJ, Vein grafts for arterial repair: An experimental study of the histological development of the intima, Ann R Coll Surg Engl, 65, pp. 85-89, (1983)
[10] Dilley RJ, McGeachie JK, Prendergast FJ, Experimental vein grafts in the rat: re‐endothelialization and permeability to albumin, Br J Surg, 70, pp. 7-12, (1983)

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