DETECTION OF FUNGAL CARBOHYDRATE ANTIGENS BY HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY USING A PROTEIN A COLUMN WITH COVALENTLY LINKED IMMUNOGLOBULIN-G

被引：6

作者：

DERUITER, GA ^{[1
]}

SMID, P ^{[1
]}

SCHOLS, HA ^{[1
]}

VANBOOM, JH ^{[1
]}

ROMBOUTS, FM ^{[1
]}

机构：

[1] GORLAEUS LABS,DEPT ORGAN CHEM,2300 RA LEIDEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 584卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80010-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fungal carbohydrate antigens were analysed by high-performance immunoaffinity chromatography (HPIAC) with immunoglobulin G (IgG) antibodies raised against extracellular polysaccharides of Mucor racemosus. The protein A-IgG complex was covalently bound with dimethyl pimelimidate, which enabled the use of strong acidic buffers to release the tightly bound antigens from the column. Prior to pulsed-amperometric detection, an anion-micromembrane suppressor was used to raise the pH of the effluent to above 12 without dilution. The HPIAC system provides a sophisticated method for the rapid and sensitive detection of antigenic oligomeric carbohydrates in biological samples and is proposed as an alternative to quantitative enzyme-linked immunosorbent assay techniques.

引用

页码：69 / 75

页数：7

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