DIETARY-REGULATION AND HORMONAL-REGULATION OF ALDOLASE-B GENE-TRANSCRIPTION IN RAT-LIVER

In the liver of the fasted rat, the aldolase B (AldB) mRNA level decreased to about half of that of the control rat. When the control rat was refed the glucose-rich diet, the AldB mRNA level increased about six to seven times more than in the fasted rat. This increase was shown as the activation of the AldB gene transcription by a nuclear run-on assay. To understand the causal factor(s) for this activation, the relationship between the AldB mRNA level in the liver and the plasma concentrations of hormones, which are known as major regulators of carbohydrate metabolism during fasting and refeeding, was investigated. The plasma insulin level in the rat which was refed the glucose-rich diet increased in parallel to AMB mRNA level, while the plasma glucagon level decreased reciprocally to it. The relationship of the plasma corticosterone level to the AldB mRNA level was not obvious. To directly confirm the effects of these hormones on AldB gene transcription in the liver, the responses of AldB gene in the primary cultured hepatocytes to these hormones were examined. Insulin and dexamethasone were effective to activate AldB gene, while glucagon and thyroxine were suppressive. Thyroxine did not extinguish the effects of insulin and dexamethasone, but glucagon canceled them. Thus, it is probable that in vivo these hormones synergistically regulate the AldB gene transcription. lit vitro transcription analysis of two AldB promoter constructs suggested that the proximal half of the AldB promoter (up to -92 bp from the transcription start site) is, at least in part, involved for this induction, and the distal half which contains liver-specific elements (-93 to -202 bp) is not involved. The possible explanation for the dietary regulation of aldolase B gene transcription in the liver is discussed. (C) 1994 Academic Press, Inc.