HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF UROCANIC ACID ISOMERS IN COSMETIC PRODUCTS

被引：5

作者：

DEORSI, D

GAGLIARDI, L

CHIMENTI, F

TONELLI, D

机构：

[1] UNIV BOLOGNA,DIPARTIMENTO CHIM IND & MAT,I-40136 BOLOGNA,ITALY

[2] IST SUPER SANITA,CHIM FARMACO LAB,I-00161 ROME,ITALY

[3] UNIV REGGIO CALABRIA,FAC FARM,I-89100 REGGIO CALABRIA,ITALY

来源：

CHROMATOGRAPHIA | 1995年 / 41卷 / 5-6期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; UROCANIC ACID ISOMERS; COSMETICS;

D O I：

10.1007/BF02688054

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the determination of trans and cis urocanic acid in oil-in-water cosmetic emulsions. It involves an extraction of the sample in 1 : 3 methanol-aqueous NaOH (10(-3) M), by ultrasonication, which leads to quantitative recoveries, and a reversed-phase HPLC isocratic elution for the analysis of the extract. Chromatography is performed on a C18 column using 0.1 M sodium perchlorate (pH 3.0)-acetonitrile 98 : 2 (v/v), as the mobile phase, and UV detection at 263 nm. The separation of the isomers is good. Linearity and precision of the method have been assessed.

引用

页码：370 / 372

页数：3

共 10 条

[1] DETERMINATION OF COMPOUND IDENTITY BY GROUP TYPE AND CLASS USING DIODE-ARRAY DETECTION IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].