A SIMPLE AND RAPID METHOD FOR THE ISOLATION OF PEPTIDES FROM SODIUM DODECYL SULFATE-CONTAINING POLYACRYLAMIDE GELS

A rapid and simple method is described for the recovery of peptides [bovine serum albumin, heart ubiquinol-cytochrome c reductase, rat cytochrome oxidase, cytochrome c] from sodium dodecyl sulfate-containing polyacrylamide gels. It involves the electrophoretic concentration of a peptide in the stacking gel followed by elution into glycerol. The method requires no special equipment or chemicals, and the elution can be made using the same electrophoretic systems used in the separation step. The method is more rapid than normal extraction procedures, and simpler than most electrophoretic elution methods described. The method can be used for isolation of microgram as well as milligram quantities of an individual peptide with yields of .apprx. 100%.