A SIMPLE AND RAPID METHOD FOR THE ISOLATION OF PEPTIDES FROM SODIUM DODECYL SULFATE-CONTAINING POLYACRYLAMIDE GELS

被引:59
作者
MENDELHARTVIG, I
机构
关键词
D O I
10.1016/0003-2697(82)90579-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid and simple method is described for the recovery of peptides [bovine serum albumin, heart ubiquinol-cytochrome c reductase, rat cytochrome oxidase, cytochrome c] from sodium dodecyl sulfate-containing polyacrylamide gels. It involves the electrophoretic concentration of a peptide in the stacking gel followed by elution into glycerol. The method requires no special equipment or chemicals, and the elution can be made using the same electrophoretic systems used in the separation step. The method is more rapid than normal extraction procedures, and simpler than most electrophoretic elution methods described. The method can be used for isolation of microgram as well as milligram quantities of an individual peptide with yields of .apprx. 100%.
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页码:215 / 217
页数:3
相关论文
共 11 条
[1]   PEPTIDE MAPPING OF PROTEINS FROM ACRYLAMIDE GELS [J].
BRAY, D ;
BROWNLEE, SM .
ANALYTICAL BIOCHEMISTRY, 1973, 55 (01) :213-221
[3]   RECOVERY OF FUNCTIONAL PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS [J].
HANAOKA, F ;
SHAW, JL ;
MUELLER, GC .
ANALYTICAL BIOCHEMISTRY, 1979, 99 (01) :170-174
[4]   2 METHODS FOR ELECTROPHORETIC ELUTION OF PROTEINS FROM POLYACRYLAMIDE GELS [J].
KARSNAS, P ;
ROOS, P .
ANALYTICAL BIOCHEMISTRY, 1977, 77 (01) :168-175
[5]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[6]   ISOELECTRIC FOCUSING OF PROTEINS EXPOSED TO SODIUM DODECYL-SULFATE [J].
MILLER, DW ;
ELGIN, SCR .
ANALYTICAL BIOCHEMISTRY, 1974, 60 (01) :142-148
[7]   A SIMPLE AND RAPID METHOD FOR THE QUANTITATIVE ISOLATION OF PROTEINS FROM POLYACRYLAMIDE GELS [J].
OTTO, M ;
SNEJDARKOVA, M .
ANALYTICAL BIOCHEMISTRY, 1981, 111 (01) :111-114
[9]   HIGH-RESOLUTION PREPARATIVE SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS - FLUORESCENT VISUALIZATION AND ELECTROPHORETIC ELUTION-CONCENTRATION OF PROTEIN BANDS [J].
STEPHENS, RE .
ANALYTICAL BIOCHEMISTRY, 1975, 65 (1-2) :369-379
[10]   PREPARATIVE ISOELECTRIC FOCUSING OF CO HEMOGLOBINS ON POLYACRYLAMIDE GELS AND CONVERSION TO THEIR OXY FORMS [J].
SUZUKI, T ;
BENESCH, RE ;
YUNG, S ;
BENESCH, R .
ANALYTICAL BIOCHEMISTRY, 1973, 55 (01) :249-254