PHOSPHOENOLPYRUVATE CARBOXYKINASE ACTIVITY IN ASCARIS SUUM MUSCLE

被引：13

作者：

DENBOSSC.H

机构：

[1] Department of Biochemistry, Janssen Pharmaceutica, Beerse

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY | 1969年 / 31卷 / 05期

关键词：

Ascaris; CO[!sub]2[!/sub] fixation; divalent ions; helminth metabolism; nucleotide co-factors; oxaloacetate; Phosphoenolpyruvate carboxykinase; sulphydryl-inhibitors;

D O I：

10.1016/0010-406X(69)92079-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Phosphoenolpyruvate (PEP) carboxykinase activity was measured for four subcellular fractions of Ascaris suum muslce. Mos activity was associated with the soluble fraction. 2. 2. The reaction had a pH optimum of about 7·2. 3. 3. Mn++ was a more effective promoter of the reaction than Mg++. 4. 4. IDP was the most effective PEP-carboxykinase co-factor; GDP was slightly less effective and ADP was much less effective. 5. 5. The presence of a sulphydryl group on the enzyme was essential for full catalytic activity. © 1969.

引用

页码：789 / +

页数：1

共 20 条

[1] STUDIES ON METABOLISM OF ECHINOCOCCUS GRANULOSUS .8. PATHWAY TO SUCCINATE IN E GRANULOSUS SCOLICES [J].