MAINTENANCE INVITRO OF ADULT FASCIOLA-HEPATICA IN A CONTINUOUS-FLOW SYSTEM

被引：5

作者：

LEHNER, RP ^{[1
]}

SEWELL, MMH ^{[1
]}

机构：

[1] UNIV EDINBURGH,DEPT TROP ANIM HLTH,EDINBURGH EH8 9YL,MIDLOTHIAN,SCOTLAND

来源：

VETERINARY PARASITOLOGY | 1979年 / 5卷 / 04期

关键词：

D O I：

10.1016/0304-4017(79)90023-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A continuous-flow apparatus is described for the maintenance of groups of adult Fasciola hepatica. The ammonia produced by the flukes was used as a measure of their metabolic activity and it was shown that this is best maintained in culture fluids containing a large proportion of bovine serum. More ammonia per fluke was consistently produced when the flow rate of the medium was increased but including small pieces of clotted sheep blood or increasing the number of flukes per culture had no significant effects. © 1979.

引用

页码：315 / 323

页数：9

共 13 条

[1]

CAVIER R, 1954, CR HEBD ACAD SCI, V238, P2448

[2]

CLEGG JA, 1957, THESIS U LONDON

[3] IMPROVED APPARATUS FOR MAINTENANCE OF SCHISTOSOMA-MANSONI AND PLASMODIUM-KNOWLESI OR OTHER BLOOD PROTOZOA IN A CONTINUOUS-FLOW MEDIUM [J].

COWPER, SG ;

MAEGRAITH, BG ;

FLETCHER, KA .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1972, 66 (01) :67-+

[4] AN ATTEMPT TO MAINTAIN SCHISTOSOMES (S MANSONI) IN A CONTINUOUS-FLOW APPARATUS [J].

COWPER, SG .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1969, 63 (02) :181-+

[5] SOME FURTHER OBSERVATIONS ON USE OF A CONTINUOUS-FLOW APPARATUS FOR MAINTENANCE OF SCHISTOSOMES AND BLOOD PROTOZOA INVITRO [J].

COWPER, SG .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1974, 68 (04) :415-&

[6] MAINTENANCE INVITRO OF FASCIOLA-HEPATICA [J].

DAWES, B .

NATURE, 1954, 174 (4431) :654-655

[7]

Earle WR, 1943, J NATL CANCER I, V4, P165

[8]

LEHNER RP, 1977, THESIS U EDINBURGH

[9]

Martinetto P, 1968, Ig Mod, V61, P864

[10] OBSERVATIONS ON THE SURVIVAL INVITRO OF BACTERIA FREE ADULT COMMON LIVER FLUKES, FASCIOLA-HEPATICA LINN, 1758 [J].

ROHRBACHER, GH .

JOURNAL OF PARASITOLOGY, 1957, 43 (01) :9-18

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