CLONING OF TLRD, A 4TH RESISTANCE GENE, FROM THE TYLOSIN PRODUCER, STREPTOMYCES-FRADIAE

被引:53
作者
ZALACAIN, M
CUNDLIFFE, E
机构
[1] UNIV LEICESTER,LEICESTER BIOCTR,LEICESTER LE1 7RH,ENGLAND
[2] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,ENGLAND
关键词
ANTIBIOTIC RESISTANCE; MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN-B ANTIBIOTICS; RIBOSOMAL-RNA METHYLATION; RECOMBINANT DNA;
D O I
10.1016/0378-1119(91)90021-3
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In addition to tlrA, tlrB and tlrC, which were previously cloned by others, a fourth antibiotic-resistance gene (tlrD) has been isolated from Streptomyces fradiae, a producer of tylosin (Ty), and cloned in Streptomyces lividans. Like tlrA, tlrD encodes an enzyme that methylates the N6-amino group of the A2058 nucleoside within 23S ribosomal RNA. However, whereas the tlrA protein dimethylates that nucleoside, the tlrD product generates N6-monomethyladenosine. The genes also differ in their mode of expression: tlrA is inducible, whereas tlrD is apparently expressed constitutively, and it has been confirmed that the tlrA-encoded enzyme can add a second methyl group to 23S rRNA that has already been monomethylated by the tlrD-encoded enzyme. Presumably, that is what happens in S. fradiae.
引用
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页码:137 / 142
页数:6
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