RECOVERY OF DECREASED ABILITY OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS FROM CHRONIC-RENAL-FAILURE TO PRODUCE INTERLEUKIN-1-ALPHA AND INTERLEUKIN-1-BETA AFTER RENAL-TRANSPLANTATION

The ability of cultured peripheral-blood mononuclear cells (PBMC) to release interleukin-1 alpha and beta (IL-1-alpha, IL-1-beta) in response to concanavalin A (con A) was investigated in patients with chronic renal failure (CRF) and in renal transplant recipients. Mean IL-1-alpha level released by PBMC of healthy subjects (n = 42), CRF patients (n = 42), or transplants 2 months after operation (n = 69) was 152 +/- 103, 110 +/- 80, or 154 +/- 87 pg/5 x 10(5) cells/ml culture, respectively. IL-1-alpha release from PBMC of recipients 2 months after renal transplantation was significantly higher than that of CRF patients (p < 0.05). Mean IL-1-beta level released by PBMC of healthy subjects (n = 34), CRF (n = 30), or transplants (n = 55) was 223 +/- 159, 135 +/- 129, or 276 +/- 155 pg/5 x 10(5) cells, respectively. Similar to IL-1-alpha, the level in CRF was significantly lower than that in healthy subjects (p < 0.05). A time course study indicated that the ability of PBMC from transplants to release IL-1-alpha and beta-promptly decreased following the operation, possibly owing to prednisolone and ciclosporin immunosuppressive therapy. However, after maintaining a low level for 2-3 weeks, IL-1 release from PBMC gradually increased thereafter. The results were consistent with known characteristics of decreased immunity in CRF states, and further suggested that the decreased ability of PBMC to release IL-1-alpha and beta in response to con A in CRF patients is recovered 2 months after renal transplantation.