MHC II-RESTRICTED, CD4+ CYTOTOXIC T-LYMPHOCYTES SPECIFIC FOR HERPES-SIMPLEX VIRUS-1 - IMPLICATIONS FOR THE DEVELOPMENT OF HERPETIC STROMAL KERATITIS IN MICE

被引:28
作者
DOYMAZ, MZ [1 ]
FOSTER, CM [1 ]
DESTEPHANO, D [1 ]
ROUSE, BT [1 ]
机构
[1] UNIV TENNESSEE,COLL VET MED,DEPT MICROBIOL,KNOXVILLE,TN 37996
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1991年 / 61卷 / 03期
关键词
D O I
10.1016/S0090-1229(05)80011-X
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Herpetic stromal keratitis (HSK) appears to represent an immunopathological reaction in which CD4+ T cells play a prominent role. However, the exact immunopathological mechanism(s) utilized by CD4+ T cells during HSK remains to be elucidated. In this study, the presence of cytotoxic CD4+ T lymphocytes in the cervical and retropharyngeal lymph nodes of Balb/c mice experiencing HSK was investigated. After in vitro depletion of CD4+ or CD8+ T cells with specific monoclonal antibodies and complement treatment, the cytotoxic functions of the remaining T cell populations were assayed by using target cells expressing either MHC Class I or both Class I and Class II. Our results showed the presence of a distinct cytotoxic T lymphocyte (CTL) population which was CD4+ and demonstrated lytic activity in a Class II-restricted fashion. Furthermore, these cells were able to develop into efficient effector CTL in the absence of CD8+ T lymphocytes as assessed by in vivo depletion experiments. Immunohistochemical methods were also utilized to show the presence of both CD4+ lymphocytes and I-A+ cells in the corneal tissues during HSK. These findings support the notion that direct lysis of infected Class II-bearing corneal cells by CD4+ CTL might be one of the mechanisms leading to stromal immunopathology in herpetic infections. © 1991 Academic Press, Inc.
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页码:398 / 409
页数:12
相关论文
共 22 条
[1]   VACCINIA VIRUS RECOMBINANT EXPRESSING HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-D PREVENTS LATENT HERPES IN MICE [J].
CREMER, KJ ;
MACKETT, M ;
WOHLENBERG, C ;
NOTKINS, AL ;
MOSS, B .
SCIENCE, 1985, 228 (4700) :737-740
[2]  
DOHERTY PC, 1990, IMMUNOL TODAY, V11, P55
[3]  
HENDRICKS RL, 1990, INVEST OPHTH VIS SCI, V31, P1929
[4]  
HENDRICKS RL, 1989, J IMMUNOL, V142, P263
[5]  
HYNDIUK RA, 1986, INFECTIONS EYE DIAGN, P343
[6]  
KAUFMAN HE, 1976, INVEST OPHTHALMOL VI, V17, P941
[7]  
KOLAITIS G, 1990, IMMUNOLOGY, V71, P101
[8]  
MARTIN S, 1988, ANN NY ACAD SCI, V352, P257
[9]  
MCBRIDE BW, 1988, IMMUNOLOGY, V65, P583
[10]   VIRAL KERATITIS [J].
MCGILL, J ;
SCOTT, GM .
BRITISH MEDICAL BULLETIN, 1985, 41 (04) :351-356