ESTIMATION OF HYDROGEN-PEROXIDE FORMED AND RESIDUAL ASCORBATE IN THE COPPER CATALYZED OXIDATION REACTION OF ASCORBATE AT PH-7

被引：4

作者：

BAKER, WL

GOODE, J

COOPER, L

机构：

[1] Chemistry Department, Swinburne Institute of Technology, Hawthorn, 3122, John Street

来源：

MIKROCHIMICA ACTA | 1992年 / 106卷 / 3-6期

关键词：

ASCORBATE; HYDROGEN PEROXIDE; COPPER; FLUORESCENT ASSAY;

D O I：

10.1007/BF01242085

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fluorescent method has been developed for determination of ascorbic acid concentrations. The method involves treatment of the ascorbic acid with Cu(II) and treating the hydrogen peroxide formed with horseradish peroxidase in the presence of p-hydroxyphenylacetic acid, to form a fluorescent p-hydroxyphenylacetic acid dimer. The reaction is suitable for analysis of concentrations in the range from 50-mu-M to 4 mM ascorbic acid solutions and can be used for analysis of pharmaceutical preparations but is unsuitable for analysis of ascorbate in preparations derived from natural sources. By using a slight modification of the analytical technique it is possible to measure the amount of hydrogen peroxide formed and the residual concentrations of ascorbic acid in solutions treated with varying amounts of copper ion.

引用

页码：143 / 152

页数：10

共 13 条

[1] DISULFIDE INHIBITION OF COPPER-CATALYZED OXIDATION OF ASCORBIC-ACID - SPECTROPHOTOMETRIC EVIDENCE FOR ACCUMULATION OF A STABLE COMPLEX [J].