EFFECT OF BUFFER COMPOSITION ON THE MIGRATION ORDER AND SEPARATION OF HISTONE H1 SUBTYPES

被引：41

作者：

LINDNER, H

HELLIGER, W

SARG, B

MERANER, C

机构：

[1] Institute of Medical Chemistry and Biochemistry, Innsbruck

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 04期

关键词：

CAPILLARY ELECTROPHORESIS; H1 HISTONE SUBTYPES; H1 HISTONE MODIFICATIONS; BUFFER COMPOSITION;

D O I：

10.1002/elps.1150160197

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of different buffer concentrations and compositions on the elution order and separation of I-Il histone subtypes and their phosphorylated modifications isolated from several species was studied using high-performance capillary electrophoresis (CE). Various cations and anions were tested in an untreated silica capillary and low pH buffers, in the presence of the dynamic coating agent hydroxypropylmethyl cellulose. It was found that the cations and anions of buffers have a remarkable influence on bath the efficiency and the selectivity of protein separations. A triethylammonium methanephosphonate system proved efficacious for the separation of rat histone subtype H1c from H1c and a perchlorate/triethylammonium phosphate system for the analysis of chicken and mouse linker histones. CE provides an attractive alternative to high-performance liquid chromatography and conventional gel electrophoresis.

引用

页码：604 / 610

页数：7

共 27 条

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