QUANTITATIVE FORMATION OF N(O,S)-HEPTAFLUOROBUTYRYL ISOBUTYL AMINO-ACIDS FOR GAS-CHROMATOGRAPHIC ANALYSIS .1. ESTERIFICATION

被引：83

作者：

MACKENZIE, SL

TENASCHUK, D

机构：

[1] Prairie Regional Laboratory, National Research Council of Canada, Saskatoon

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 171卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(01)95299-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effect of temperature, reaction time and HCL concentration on the formation of the isobutyl esters of protein amino acids has been studied. Quantitative esterification is achieved by heating at 120° for 30 min in 3 M HCl-isobutanol. Experimental procedures have been examined in detail and potential sources of error in derivatisation and chromatography have been identified. © 1979.

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页码：195 / 208

页数：14

相关论文

共 26 条

[1] DETERMINATION OF AMINO-ACID PROFILES IN BIOLOGICAL SAMPLES BY GAS-CHROMATOGRAPHY [J].

ADAMS, RF .

JOURNAL OF CHROMATOGRAPHY, 1974, 95 (02) :189-212

[2] ULTRAMICRO GC DETERMINATION OF AMINO-ACIDS USING GLASS OPEN TUBULAR COLUMNS AND A NITROGEN-SELECTIVE DETECTOR [J].

ADAMS, RF ;

VANDEMARK, FL ;

SCHMIDT, GJ .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1977, 15 (02) :63-68

[3] IMPROVED PROCEDURE FOR PREPARING N-BUTYL-TRIFLUOROACETYL AMINO-ACID DERIVATIVE AND ITS APPLICATION IN STUDY OF RADIOACTIVE AMINO-ACIDS FROM BIOLOGICAL SOURCES [J].

CANCALON, P ;

KLINGMAN, JD .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1974, 12 (06) :349-349

[4] QUANTITATIVE GAS-LIQUID-CHROMATOGRAPHY AND MASS-SPECTROMETRY OF N(O)-PERFLUOROBUTYRYL-O-ISOAMYL DERIVATIVES OF AMINO-ACIDS [J].

FELKER, P ;

BANDURSKI, RS .

ANALYTICAL BIOCHEMISTRY, 1975, 67 (01) :245-262

[5] GAS-LIQUID-CHROMATOGRAPHY OF HEPTAFLUOROBUTYRYL-O-ISOBUTYL ESTERS OF AMINO-ACIDS [J].

FELKER, P .

JOURNAL OF CHROMATOGRAPHY, 1978, 153 (01) :259-262

[6] TRIMETHYLSILYLATION OF AMINO ACIDS - DERIVATIZATION AND CHROMATOGRAPHY [J].

GEHRKE, CW ;

LEIMER, K .

JOURNAL OF CHROMATOGRAPHY, 1971, 57 (02) :219-&

[7] CHROMATOGRAPHIC SEPARATION OF N-BUTYL N-TRIFLUOROACETYL ESTERS OF AMINO ACIDS [J].

GEHRKE, CW ;

SHAHROKHI, F .

ANALYTICAL BIOCHEMISTRY, 1966, 15 (01) :97-+

[8]

GEHRKE CW, 1973, J CHROMATOGR, V76, P63, DOI 10.1016/S0021-9673(01)97778-7

[9] GAS-LIQUID CHROMATOGRAPHIC ANALYSIS OF TRYPTOPHAN IN PROTEINS [J].

GEHRKE, CW ;

TAKEDA, H .

JOURNAL OF CHROMATOGRAPHY, 1973, 76 (01) :77-89

[10]

HARRIS WE, 1966, PROGRAMMED TEMPERATU