PRODUCTION AND CHARACTERIZATION OF ANTIBODY AGAINST AFLATOXIN-M1

被引：43

作者：

HARDER, WO

CHU, FS

机构：

[1] Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, 53706, Wisconsin

来源：

EXPERIENTIA | 1979年 / 35卷 / 08期

关键词：

D O I：

10.1007/BF01949970

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antibody against aflatoxin M1 was obtained after immunization of rabbits with bovine serum albumin-afla M1 oxime conjugate. The antibody has greatest binding efficiency for afla M1, and was less efficient for afla B1. Cross-reaction of antibody with aflatoxin Q1, aflatoxicol, and aflatoxin B2a was weak. Aflatoxin B2, G1, and G2 and afla B1-guanine adduct showed almost no cross-reaction with the antibody. The sensitivity of the binding assay for aflatoxin M1 detection is in the range of 1-10 ng per assay. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody determination are described. © 1979 Birkhäuser Verlag.

引用

页码：1104 / 1107

页数：4

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