An Electron Paramagnetic Resonance Study of Conformational Substate Distribution in High Spin Heme-Proteins

被引:8
作者
Bizzarri, A. R. [1 ,2 ]
Cannistraro, S. [1 ]
机构
[1] Dipartimento Fis Univ, Grp Biofis Mol, I-06100 Perugia, Italy
[2] ISAS Int Sch Adv Studies, Trieste, Italy
关键词
D O I
10.1007/BF03166071
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
The electron paramagnetic resonance spectra of high spin ferric horse myoglobin and human hemoglobin have been analyzed by computer simulation in terms of a distribution of the metal ion crystal field energies Delta(1) and Delta(2). The widths of these distributions have been put into relation to the distribution of the conformational substates which may be sampled by the biomolecules during their physiological activity and become eventually arrested at low temperature. These distributions are found to be dependent upon both the solvent used and the cooling history to which the samples were submitted. The results are discussed in connection with the physical analogies displayed by these biomolecules and the glassy systems in general.
引用
收藏
页码:627 / 645
页数:19
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