EXON STRUCTURE AT THE HUMAN ACP1 LOCUS SUPPORTS ALTERNATIVE SPLICING MODEL FOR F-ISOZYME AND S-ISOZYME GENERATION

被引：41

作者：

LAZARUK, KDA ^{[1
]}

DISSING, J ^{[1
]}

SENSABAUGH, GF ^{[1
]}

机构：

[1] UNIV COPENHAGEN,INST FORENS GENET,DK-1168 COPENHAGEN,DENMARK

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2269

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human ACP1 locus encodes a genetically polymorphic cytoplasmic low-molecular-weight acid phosphatase. Each of the common alleles encodes two isoforms, f and s. Both isozymes are of equal length (157 residues) but differ in sequence over an internal 34 residue segment. Substantial portions of the ACP1*A, *B and *C alleles common to Europeans have been sequenced. Six linearly positioned exons containing codons 14 to 157 were identified. Two exons of equal length (114bp) interspaced by a short (41bp), probably nonfunctional, intron encode the specific f and s segments, respectively. These findings strongly support an alternative RNA splicing hypothesis. In addition, three allele-specific base substitutions were encountered. © 1993 Academic Press. All rights reserved.

引用

页码：440 / 446

页数：7

共 40 条

[1] HUMAN CARBONIC ANHYDRASE-I CDNA [J].

BARLOW, JH ;

LOWE, N ;

EDWARDS, YH ;

BUTTERWORTH, PHW .

NUCLEIC ACIDS RESEARCH, 1987, 15 (05) :2386-2386

[2] RESOLUTION OF THE LOW-MOLECULAR-WEIGHT ACID-PHOSPHATASE IN AVIAN PECTORAL MUSCLE INTO 2 DISTINCT ENZYME FORMS [J].

BAXTER, JH ;

SUELTER, CH .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 239 (01) :29-37

[3]

BLAKE NM, 1973, JPN J HUM GENET, V18, P10

[4] STUDIES OF IN-VITRO EFFECTS OF OXIDIZED GLUTATHIONE AND ACETYLPHENYLHYDRAZINE ON ACID PHOSPHATASES OF HUMAN RED BLOOD CELL - EXPERIMENTAL MODEL FOR INVESTIGATION OF HEMOLYTIC DRUG ACTION AT MOLECULAR LEVEL [J].

BOTTINI, E ;

MODIANO, G ;

SANTOLAM.C ;

FILIPPI, G ;

BUSINCO, L .

CLINICA CHIMICA ACTA, 1971, 31 (01) :243-&

[5] MOLECULAR-CLONING OF PROTEIN 4.1, A MAJOR STRUCTURAL ELEMENT OF THE HUMAN-ERYTHROCYTE MEMBRANE SKELETON [J].

CONBOY, J ;

KAN, YW ;

SHOHET, SB ;

MOHANDAS, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9512-9516

[6]

COOPER TA, 1992, J BIOL CHEM, V267, P5330

[7] IMMUNOCHEMICAL CHARACTERIZATION OF HUMAN RED-CELL ACID-PHOSPHATASE ISOZYMES [J].

DISSING, J .

BIOCHEMICAL GENETICS, 1987, 25 (11-12) :901-918

[8] HUMAN RED-CELL ACID-PHOSPHATASE (ACP1) - EVIDENCE FOR DIFFERENCES IN THE PRIMARY STRUCTURE OF THE 2 ISOZYMES ENCODED BY THE ACP1-]B ALLELE [J].

DISSING, J ;

SENSABAUGH, GF .

BIOCHEMICAL GENETICS, 1987, 25 (11-12) :919-927

[9] PHOSPHONIC AND ARSONIC ACIDS AS INHIBITORS OF HUMAN RED-CELL ACID-PHOSPHATASE AND THEIR USE IN AFFINITY CHROMATOGRAPHY [J].

DISSING, J ;

DAHL, O ;

SVENSMARK, O .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 569 (02) :159-176

[10] HUMAN RED-CELL ACID-PHOSPHATASE (ACP1) - THE PRIMARY STRUCTURE OF THE 2 PAIRS OF ISOZYMES ENCODED BY THE ACP1-ASTERISK-A AND ACP1-ASTERISK-C ALLELES [J].

DISSING, J ;

JOHNSEN, AH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1121 (03) :261-268

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