CAPILLARY MICELLAR ELECTROKINETIC, SEQUENTIAL MULTIWAVELENGTH CHROMATOGRAPHIC CHARACTERIZATION OF A CHIMERIC MONOCLONAL-ANTIBODY CYTOTOXIN CONJUGATE

被引：19

作者：

HUGHES, DE

RICHBERG, P

机构：

[1] Bristol-Myers Squibb Company, Pharamaceutical Research Institute, Syracuse, NY 13221-4755

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 635卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80374-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An analysis of a doxorubicin-conjugated chimeric antibody has been developed by micellar electrokinetic capillary chromatography. The procedure separates the conjugated antibody, the antibody light and heavy chains, antibody fragments, and unconjugated doxorubicin. The protein-derived species are monitored at 280 nm and the doxorubicin bearing species are monitored at 500 nm. Consistency with the proposed in vivo mechanism of action of the antibody conjugate in acid media is demonstrated.

引用

页码：313 / 318

页数：6

共 14 条

[1]

ARCAMONE F, 1961, GIORN MICROBIOL, V9, P83

[2]

ARCAMONE F, 1981, DOXORUBICIN ANTICANC, P17

[3] ADRIAMYCIN(HYDRAZONE)-ANTIBODY CONJUGATES REQUIRE INTERNALIZATION AND INTRACELLULAR ACID-HYDROLYSIS FOR ANTITUMOR-ACTIVITY [J].