DNA TOPOISOMERASE-2-BETA - STABILITY AND DISTRIBUTION IN DIFFERENT ANIMAL-CELLS IN COMPARISON TO DNA TOPOISOMERASE-I AND TOPOISOMERASE-II-ALPHA

被引：21

作者：

NEGRI, C ^{[1
]}

SCOVASSI, AI ^{[1
]}

BRAGHETTI, A ^{[1
]}

GUANO, F ^{[1
]}

RICOTTI, GCBA ^{[1
]}

机构：

[1] CNR,IST GENET BIOCHIM & EVOLUZ,VIA ABBIATEGRASSO 207,I-27100 PAVIA,ITALY

来源：

EXPERIMENTAL CELL RESEARCH | 1993年 / 206卷 / 01期

关键词：

D O I：

10.1006/excr.1993.1128

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DNA topoisomerase I and the isoforms α and β of DNA topoisomerase II were analyzed in different animal cells using a panel of monoclonal antibodies (MoAbs). The β isoform is a most unstable enzyme. We investigated conditions to stabilize β isoform because its variability changes according to the derivation of cells. We describe two MoAbs specific to DNA topoisomerase I: the first one recognizes the enzyme in all the species tested including fish; the second one, in contrast, recognizes an epitope present only in mammalian cells. We also found that eight of eight MoAbs against DNA topoisomerase II α and five of six against the β isoform recognize the respective enzymes in all the species tested excluding fish. In addition, MoAbs to the α isoform are specific to epitopes not present in the carboxyl third of the enzyme. © 1993 Academic Press, Inc.

引用

页码：128 / 133

页数：6

共 17 条

[1]

CHUNG TD, 1989, P NATL ACAD SCI USA, V86, P9341

[2]

DRAKE FH, 1987, J BIOL CHEM, V262, P16739

[3] BIOCHEMICAL AND PHARMACOLOGICAL PROPERTIES OF P170 AND P180 FORMS OF TOPOISOMERASE-II [J].

DRAKE, FH ;

HOFMANN, GA ;

BARTUS, HF ;

MATTERN, MR ;

CROOKE, ST ;

MIRABELLI, CK .

BIOCHEMISTRY, 1989, 28 (20) :8154-8160

[4]

EARNSHAW WC, 1988, CANCER CELL, V6, P279

[5]

FRY AJ, UNPUB

[6] COHABITATION OF SCAFFOLD BINDING REGIONS WITH UPSTREAM ENHANCER ELEMENTS OF 3 DEVELOPMENTALLY REGULATED GENES OF DROSOPHILA-MELANOGASTER [J].

GASSER, SM ;

LAEMMLI, UK .

CELL, 1986, 46 (04) :521-530

[7] DIFFERENTIAL EXPRESSION OF DNA TOPOISOMERASES-I AND TOPOISOMERASES-II DURING THE EUKARYOTIC CELL-CYCLE [J].

HECK, MMS ;

HITTELMAN, WN ;

EARNSHAW, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (04) :1086-1090

[8]

HSIANG YH, 1988, CANCER RES, V48, P3230

[9]

HWANG JL, 1989, CANCER RES, V49, P958

[10]

KAUFMANN SH, 1991, CANCER RES, V51, P3534

← 1 2 →