MUTATIONAL ANALYSIS OF THE BACTERIOPHAGE-P2 OGR PROTEIN - TRUNCATION OF THE CARBOXY-TERMINUS

被引:5
作者
GEBHARDT, K
KING, RA
CHRISTIE, GE
LINDQVIST, BH
机构
[1] UNIV OSLO,INST BIOL,N-0317 OSLO 3,NORWAY
[2] UNIV OSLO,BIOTECHNOL CTR OSLO,N-0317 OSLO 3,NORWAY
[3] VIRGINIA COMMONWEALTH UNIV,DEPT MICROBIOL & IMMUNOL,RICHMOND,VA 23298
关键词
D O I
10.1128/JB.175.23.7724-7726.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Ogr protein is a 72-residue, zinc-binding transcription factor essential for activation of late gene expression in bacteriophage P2. Analysis of C-terminal truncated proteins generated by stop codon mutagenesis shows that deletion of residues distal to position 51 had negligible effects on Ogr function. More-extensive deletion resulted in unstable products with severely reduced activity. These results, as well as the effects of other mutations in this region, support the idea that the 21 C-terminal residues are not required for transactivation.
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页码:7724 / 7726
页数:3
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