STIMULATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MESSENGER-RNA LEVELS BY ENDOGENOUS NITRIC-OXIDE IN CYTOKINE-ACTIVATED ENDOTHELIUM

被引：48

作者：

BERETA, J

BERETA, M

机构：

[1] Department of Biochemistry, Jagiellonian University, Cracow

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 217卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.2785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have shown that endogenous nitric oxide (NO) potentiates glycolysis in the cytokine-activated murine microvascular endothelial cells (MME). In the present study we investigate the influence of NO on the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an enzyme of the glycolytic pathway. Activation of MME with TNF-alpha and IFN-gamma resulted in a strong elevation of GAPDH mRNA levels. This effect was impaired in the presence of L-NMMA, the inhibitor of NO synthesis. We discuss the possibility that NO-mediated elevation of GAPDH mRNA levels may compensate for NO-mediated inhibition of GAPDH enzymatic activity, representing another adaptive mechanism which protects cells producing large amounts of NO against its cytotoxic effects. (C), 1995 Academic Press. Inc.

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收藏

页码：363 / 369

页数：7

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