GENOTYPING OF THE GRANULOCYTE-SPECIFIC NA ANTIGENS FROM SMALL QUANTITIES OF BLOOD OR SERUM

被引：10

作者：

STEIN, EL ^{[1
]}

SANTOSO, S ^{[1
]}

BEHRENS, G ^{[1
]}

MUELLERECKHARDT, C ^{[1
]}

BUX, J ^{[1
]}

机构：

[1] UNIV GIESSEN,INST CLIN IMMUNOL & TRANSFUS MED,D-35385 GIESSEN,GERMANY

来源：

TISSUE ANTIGENS | 1995年 / 45卷 / 01期

关键词：

GRANULOCYTES; NEUTROPHILS; DNA TYPING; ANTIGENS;

D O I：

10.1111/j.1399-0039.1995.tb02417.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To avoid the well-known shortcomings of phenotyping granulocytes for the NA antigens using NA-specific human sera, a DNA-based method to determine the NA genotype was developed. Genomic DNA was isolated from blood cells or serum, amplified by polymerase chain reaction (PCR), immobilized on nylon membrane and genotyped using digoxigenin-labeled, sequence-specific oligonucleotides (SSO). The genotyping results of whole blood samples from 54 and of serum from 20 individuals correlated perfectly with our phenotyping using the antigen capture assay MAIGA. In three cases with the phenotype ''NA-null'' no hybridization of the NA-specific oligonucleotides occurred. These data show that SSO is a reliable method for NA genotyping especially if only small volumes of blood or even only serum probes are available.

引用

页码：69 / 72

页数：4

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