CLONING AND CHARACTERIZATION OF HYDROGENASE GENES FROM AZOTOBACTER-CHROOCOCCUM

被引：16

作者：

TIBELIUS, KH ^{[1
]}

ROBSON, RL ^{[1
]}

YATES, MG ^{[1
]}

机构：

[1] UNIV SUSSEX, AFRC, NITROGEN FIXAT UNIT, BRIGHTON BN1 9RQ, E SUSSEX, ENGLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1987年 / 206卷 / 02期

关键词：

D O I：

10.1007/BF00333586

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic DNA from Azotobacter chroococcum was shown by DNA hybridization to contain sequences homologous to Rhizobium japonicum H2-uptake (hup) hydrogenase genes carried on the plasmid pHU1. Two recombinant cosmid clones, pACD101 and pACD102, were isolated from a gene library of A. chroococcum by colony hybridization and physically mapped. Each contained approximately 42 kb of insert DNA with approximately 27 kb of overlapping DNA. Further hybridization studies using three fragments from pHU1 (6 kb HindIII, 6.4 kb Bg/II and 5 kb EcoRI) showed that the hup-specific regions of R. japonicum and A. chroococcum are probably highly conserved. Weak homology to the hydrogenase structural genes from Desulfovibrio vulgaris (Hildenborough) was also observed. A 24 kb BamHI fragment from pACD102 subcloned into a broad host-range vector restored hydrogenase activity to several Hup- mutants of A. chroococcum.

引用

页码：285 / 290

页数：6

共 33 条

[1]

AGUILAR OM, 1985, J GEN MICROBIOL, V131, P3141

[2] MOLECULAR AND IMMUNOLOGICAL COMPARISON OF MEMBRANE-BOUND, H-2-OXIDIZING HYDROGENASES OF BRADYRHIZOBIUM-JAPONICUM, ALCALIGENES-EUTROPHUS, ALCALIGENES-LATUS, AND AZOTOBACTER-VINELANDII [J].

ARP, DJ ;

MCCOLLUM, LC ;

SEEFELDT, LC .

JOURNAL OF BACTERIOLOGY, 1985, 163 (01) :15-20

[3]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[4] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[5] CO-TRANSFER OF DETERMINANTS FOR HYDROGENASE ACTIVITY AND NODULATION ABILITY IN RHIZOBIUM-LEGUMINOSARUM [J].

BREWIN, NJ ;

DEJONG, TM ;

PHILLIPS, DA ;

JOHNSTON, AWB .

NATURE, 1980, 288 (5786) :77-79

[6] CONSTRUCTION OF A RHIZOBIUM-JAPONICUM GENE BANK AND USE IN ISOLATION OF A HYDROGEN UPTAKE GENE [J].

CANTRELL, MA ;

HAUGLAND, RA ;

EVANS, HJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :181-185

[7] IDENTIFICATION AND CHARACTERIZATION OF PLASMIDS IN HYDROGEN UPTAKE POSITIVE AND HYDROGEN UPTAKE NEGATIVE STRAINS OF RHIZOBIUM-JAPONICUM [J].

CANTRELL, MA ;

HICKOK, RE ;

EVANS, HJ .

ARCHIVES OF MICROBIOLOGY, 1982, 131 (02) :102-106

[8]

CROSVELD FG, 1982, NUCLEIC ACIDS RES, V10, P6715

[9] PLASMIDS RELATED TO THE BROAD HOST RANGE VECTOR, PRK290, USEFUL FOR GENE CLONING AND FOR MONITORING GENE-EXPRESSION [J].

DITTA, G ;

SCHMIDHAUSER, T ;

YAKOBSON, E ;

LU, P ;

LIANG, XW ;

FINLAY, DR ;

GUINEY, D ;

HELINSKI, DR .

PLASMID, 1985, 13 (02) :149-153

[10] ASPECTS OF HYDROGEN METABOLISM IN NITROGEN-FIXING LEGUMES AND OTHER PLANT-MICROBE ASSOCIATIONS [J].

EISBRENNER, G ;

EVANS, HJ .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1983, 34 :105-136

← 1 2 3 4 →