ANALYSIS OF INSECT JUVENILE HORMONES BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY - PROBLEMS OF SAMPLE PREPARATION AND CHOICE OF DETECTION PROCEDURE

被引：14

作者：

MAUCHAMP, B

LAFONT, R

HARDY, M

JOURDAIN, D

机构：

[1] ECOLE NORM SUPER, ZOOL LAB, F-75230 PARIS 05, FRANCE

[2] LAB APPLICAT RIBERMAG, F-92502 RUEIL MALMAISON, FRANCE

来源：

BIOMEDICAL MASS SPECTROMETRY | 1979年 / 6卷 / 07期

关键词：

D O I：

10.1002/bms.1200060703

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper we introduce a novel procedure for the analysis of insect juvenile hormones and describe the principal steps involved in this method. Due to the low concentration of these hormones in insects, careful purification of samples before analysis is necessary. Biological samples are purified by solvent partition and reversed phase high pressure liquid chromatography, then analysed by gas chromatography mass spectrometry using a spectrometer equipped with a data acquisition system. By this procedure 100 pg of insect juvenile hormone could be detected in biological extracts. Copyright © 1979 Heyden & Son Ltd

引用

页码：276 / 281

页数：6

共 24 条

[1] SIMPLE AND SENSITIVE RADIOIMMUNOASSAY OF INSECT JUVENILE HORMONE USING AN IODINATED TRACER [J].