DIFFERENTIAL STAINING OF COBALT-FILLED AND NICKEL-FILLED NEURONS USING RUBEANIC ACID

被引：108

作者：

QUICKE, DLJ

BRACE, RC

机构：

[1] Department of Zoology, University of Nottingham, Nottingham, NG7 2RD, University Park

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1979年 / 115卷 / MAR期

关键词：

D O I：

10.1111/j.1365-2818.1979.tb00165.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A staining procedure is described which differentiates with distinct colours, cobalt and nickel ions introduced into neurones. Densely coloured precipitates are produced with these ions when rubeanic acid, a spot‐test reagent, is added. Formation of the coloured complexes takes place within a few minutes, and may be followed under the dissecting microscope. This technique permits observation of the relative positions, within ganglia, of somata related to two emergent nerves following multiple axonal‐backfilling. It also appears to be of value in tracing neurone branching after the intracellular injection of ions. 1979 Blackwell Science Ltd

引用

页码：161 / &

共 5 条

[1]

FIEGL F, 1947, QUALITATIVE ANAL SPO

[2]

Kater S. B., 1973, INTRACELLULAR STAINI

[3] BRANCHING OF CENTRAL NEURONS - INTRACELLULAR COBALT INJECTION FOR LIGHT AND ELECTRON-MICROSCOPY [J].