STUDIES ON BACILLUS-THURINGIENSIS H-14 STRAINS ISOLATED IN EGYPT .2. ULTRASTRUCTURE STUDIES

被引:2
作者
ABDELHAMEED, A [1 ]
LOUNATMAA, K [1 ]
CARLBERG, G [1 ]
ELTAYEB, OM [1 ]
机构
[1] UNIV CAIRO,FAC PHARM,DEPT MICROBIOL,CAIRO,EGYPT
关键词
D O I
10.1007/BF01201302
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Electron microscopy of thin sections of two mosqulto-toxic isolates of Bacillus thuringiensis H-14 showed inlcusions that varied in shape, size and stainability. The shape of some of these inclusions have not been reported before. Uitrastructural analysis of sporulation and parasporal crystal development shows that the insecticidal inclusions are initiated at the stage of axial filament formation during sporulation. In most of the inclusions there is a common subunit, formed first, which has a regular geometrical shape (rhomboid, hexagonal rectangular, trapezoid or pentagonal). Later added sub-units are more amorphous and this leads to a variety of inclusion shapes which appear at the end of sporulation. Some of these reguiar moleties develop in different ways, leading to cubical, rhomboid or parallelogramic-shaped crystais. In a freeze-tractured cell, the internal fracture faces of a crystal are seen. The size of the sub-units of this crystal was about 5×5 nm. For French summary, see next page. © 1990 Rapid Communications of Oxford Ltd.
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页码:305 / 312
页数:8
相关论文
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