CULTURE CONFIRMATION AND TYPING OF HERPES-SIMPLEX VIRUS ISOLATES FROM CLINICAL SPECIMENS WITH NEW MONOCLONAL-ANTIBODY TYPING REAGENTS

被引：2

作者：

JOHNSTON, SLG ^{[1
]}

GLEAVES, CA ^{[1
]}

机构：

[1] BELLIN MEM HOSP,DEPT LAB MED,GREEN BAY,WI

来源：

SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE | 1990年 / 4卷 / 04期

关键词：

HERPES SIMPLEX VIRUS; MONOCLONAL ANTIBODIES; CELL CULTURE; IMMUNOFLUORESCENCE;

D O I：

10.1016/0888-0786(90)90017-I

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

FITC-labelled monoclonal antibody typing reagents for herpes simplex virus (HSV) (PathoDx, Diagnostic Products Corp., Los Angeles, California) were compared to another commercially available FITC-labelled monoclonal antibody typing reagent for culture confirmation and typing of HSV isolates from clinical specimens. A total of 344 clinical specimens were evaluated for culture confirmation and typing by the two systems. There were 296 HSV-positive cultures identified by cytopathic effect (CPE). The PathoDx typing reagents identified 143 isolates as HSV-1, and 151 isolates as HSV-2, with two isolates staining with both the type 1 and type 2 reagent. The PathoDx typing reagents had a 100% concordance with the other set of typing reagents. © 1990.

引用

页码：295 / 297

页数：3

共 10 条

[1]

Corey, The diagnosis and treatment of genital herpes, JAMA, 248, pp. 1041-1049, (1982)

[2]

Corey, Laboratory diagnosis of herpes simplex virus infections principles guiding the development of rapid diagnostic test, Diagnostic Microbiology and Infectious Disease, 4, pp. 111S-119S, (1986)

[3]

Lafferty, Krofft, Remington, Et al., Diagnosis of herpes simplex virus by direct immunofluorescence and viral isolation from samples of external genital lesions in a high-prevalence population, J Clin Microbiol, 25, pp. 323-326, (1987)

[4]

Gleaves, Rice, Bindra, Hursh, Curtis, Lee, Wendt, Evaluation of a HSV specific monoclonal antibody reagent for laboratory diagnosis of herpes simplex virus infection, Diagn Microbiol Infect Dis, 12, pp. 315-318, (1989)

[5]

Aarnaes, Peterson, de la Maza, Evaluation of a new herpes simplex virus typing reagent for tissue culture confirmation, Diagn Microbiol Infect Dis, 12, pp. 269-270, (1989)

[6]

Gleaves, Swierkosz, Lee, Meyers, Serological confirmation and typing of clinical herpes simplex virus isolates: comparison of two different monoclonal antibody immunofluorescence kits, Serodiag Immunother Infect Dis, 2, pp. 151-156, (1988)

[7]

Pereira, Dondero, Gallo, Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies, Infect Immun, 35, pp. 363-367, (1982)

[8]

Peterson, Aarnaes, Bryan, Ruth, de la Maza, Typing of herpes simplex virus with synthetic DNA probes, J Infect Dis, 153, pp. 757-762, (1986)

[9]

Swierkosz, Arens, Schmidt, Armstrong, Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus, J Clin Microbiol, 21, pp. 643-644, (1985)

[10]

Peterson, Schmidt, Goldstein, Nowinski, Corey, Typing of clinical herpes simplex virus isolates with mouse monoclonal antibodies to herpes simplex virus types 1 and 2: Comparison with type-specific rabbit antisera and restriction endonuclease analysis of viral DNA, J Clin Microbiol, 17, pp. 92-96, (1983)

← 1 →