CONSTRUCTION AND CHARACTERIZATION OF A NOTL-BSUE LINKING LIBRARY FROM THE HUMAN X-CHROMOSOME

被引：8

作者：

ARENSTORF, HP ^{[1
]}

KANDPAL, RP ^{[1
]}

BASKARAN, N ^{[1
]}

PARIMOO, S ^{[1
]}

TANAKA, Y ^{[1
]}

KITAJIMA, S ^{[1
]}

YASUKOCHI, Y ^{[1
]}

WEISSMAN, SM ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV,MED RES INST,TOKYO 113,JAPAN

来源：

GENOMICS | 1991年 / 11卷 / 01期

关键词：

D O I：

10.1016/0888-7543(91)90108-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe the construction and characterization of methylation-resistant sequence-tagged NotI linking clones specific for the X chromosome, referred to as NotI-BsuE linking clones. The approach consists of methylating the X-chromosome-specific cloned DNA with BsuE methylase (M. BsuE), an enzyme that methylates the first C residue in the CGCG sequence, followed by selection of the methylation-resistant NotI sites by insertion of a kanamycin-resistance gene in the clones cleavable by NotI. The frequent occurrence of NotI sites in CpG islands is expected to cause methylation of a large number of NotI sites with BsuE methylase, thereby rendering them resistant to NotI cleavage. Thus, the combination of M. BsuE and NotI yields less frequent cutting than the NotI alone. We have isolated, partially squenced, and characterized 113 NotI-BsuE linking clones, and mapped 50 clones to various regions along the chromosome. © 1991.

引用

页码：115 / 123

页数：9

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[1] RELATIONSHIP BETWEEN PROMOTER STRUCTURE AND TEMPLATE SPECIFICITIES EXHIBITED BY THE BACTERIOPHAGE T3 AND T7 RNA-POLYMERASES [J].