FUNDAMENTAL CHROMATOGRAPHIC PARAMETERS IN COUNTERCURRENT CHROMATOGRAPHY - INFLUENCE OF THE VOLUME OF STATIONARY PHASE AND THE FLOW-RATE

Two CCC (Countercurrent Chromatography) devices were compared using a test mixture of saturated fatty acids (myristic, palmitic and stearic acids) and the two-phase heptane/acetic acid/methanol solvent system. One device, defined by Dr. ITO as a HDES Type J, is known as a HSCCC (High Speed CCC) apparatus and the other one is defined as a CDCCC (Centrifugal Droplet CCC) HSES. The influence of the rotational speed and the flow-rate on the retention of stationary phase was studied. The variations of capacity factors and resolution against the retention of stationary phase were then plotted and we checked that conventional chromatographic equations can be applied to these curves. Finally a comparison was held between the plots of various chromatographic parameters against the flow-rate between each apparatus and also the ordinary chromatography curves. We also compared the calculated partition coefficients for the fatty acids on each device with the values obtained by the "Dual Mode" method on the CDCCC apparatus and by a scintillation detector used with radioactive fatty acids.